Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055325                                                              17    2528
NP_149015                                                              17    2528
XP_006715989                                                           17    2528
NP_005323                                                              17    4312
XP_005255345                                                           17    4312
XP_005255344                                                           17    4312
NP_003698                                                              16    5631
NP_001162593                                                           15    9605
NP_002016                                                              15    9605
NP_001162594                                                           15    9605
NP_001162595                                                           15    9605
NP_001162596                                                           15    9605
NP_001164099                                                           15    9605
NP_689412                                                              15    9605
NP_689410                                                              15    9605
NP_006231                                                              15    9605
XP_005274610                                                           15    9605
>NP_055325
          Length = 4935

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LSTTKTKRT 9
            LSTTK+ RT
Sbjct: 2900 LSTTKSHRT 2908
>NP_149015
          Length = 5142

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LSTTKTKRT 9
            LSTTK+ RT
Sbjct: 2900 LSTTKSHRT 2908
>XP_006715989
          Length = 5148

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    LSTTKTKRT 9
            LSTTK+ RT
Sbjct: 2900 LSTTKSHRT 2908
>NP_005323
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 LSTTKTKRT 9
         +S TKT+RT
Sbjct: 1 MSLTKTERT 9
>XP_005255345
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 LSTTKTKRT 9
         +S TKT+RT
Sbjct: 1 MSLTKTERT 9
>XP_005255344
          Length = 154

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LSTTKTKRT 9
          +S TKT+RT
Sbjct: 13 MSLTKTERT 21
>NP_003698
          Length = 456

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  STTKTKR 8
          STTKT+R
Sbjct: 8  STTKTQR 14
>NP_001162593
          Length = 1276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSTTKTKRT 9
           +++TK KRT
Sbjct: 916 ITSTKPKRT 924
>NP_002016
          Length = 1311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSTTKTKRT 9
           +++TK KRT
Sbjct: 949 ITSTKPKRT 957
>NP_001162594
          Length = 1301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSTTKTKRT 9
           +++TK KRT
Sbjct: 939 ITSTKPKRT 947
>NP_001162595
          Length = 1276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSTTKTKRT 9
           +++TK KRT
Sbjct: 914 ITSTKPKRT 922
>NP_001162596
          Length = 1272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSTTKTKRT 9
           +++TK KRT
Sbjct: 910 ITSTKPKRT 918
>NP_001164099
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSTTKTKRT 9
           +++TK KRT
Sbjct: 590 ITSTKPKRT 598
>NP_689412
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STTKTKRT 9
          S+TKT+R+
Sbjct: 6  SSTKTRRS 13
>NP_689410
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STTKTKRT 9
          S+TKT+R+
Sbjct: 6  SSTKTRRS 13
>NP_006231
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STTKTKRT 9
          S+TKT+R+
Sbjct: 6  SSTKTRRS 13
>XP_005274610
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  STTKTKRT 9
          S+TKT+R+
Sbjct: 6  SSTKTRRS 13
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.103    0.240 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,407
Number of extensions: 466
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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