Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
XP_006715292                                                           18    1135
XP_006726157                                                           16    5631
XP_005275612                                                           16    5631
XP_005275610                                                           16    5631
XP_006726064                                                           16    5631
XP_005275460                                                           16    5631
XP_006715157                                                           16    5631
XP_005249149                                                           16    5631
NP_002254                                                              16    5631
NP_001129673                                                           16    7355
NP_060661                                                              16    7355
NP_001129674                                                           16    7355
NP_078986                                                              16    7355
NP_001273700                                                           16    7355
NP_001183959                                                           16    7355
NP_115593                                                              16    7355
NP_078944                                                              16    7355
NP_942592                                                              16    7355
NP_060217                                                              16    7355
NP_065741                                                              16    7355
XP_005259322                                                           16    7355
XP_005258982                                                           16    7355
XP_005266498                                                           16    7355
XP_005265730                                                           16    7355
XP_005265729                                                           16    7355
XP_005265728                                                           16    7355
XP_005265724                                                           16    7355
NP_001099040                                                           16    7355
NP_001116                                                              16    7355
NP_005583                                                              15    9605
NP_001159752                                                           15    9605
NP_001159753                                                           15    9605
NP_937784                                                              15    9605
NP_061870                                                              15    9605
NP_114107                                                              15    9605
NP_653213                                                              15    9605
XP_006722447                                                           15    9605
XP_005252053                                                           15    9605
XP_005252052                                                           15    9605
XP_005252051                                                           15    9605
>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LSTADG VE
Sbjct: 462 LSTADGRVE 470
>XP_006715292
          Length = 759

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LSTADG VE
Sbjct: 457 LSTADGRVE 465
>XP_006726157
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>XP_005275612
          Length = 633

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 179 LSTQEGLVQ 187
>XP_005275610
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>XP_006726064
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>XP_005275460
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>XP_006715157
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>XP_005249149
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>NP_002254
          Length = 673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSTADGLVE 9
           LST +GLV+
Sbjct: 220 LSTQEGLVQ 228
>NP_001129673
          Length = 205

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LSTADGLV 8
           LST++GL+
Sbjct: 158 LSTSEGLI 165
>NP_060661
          Length = 531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSTADGLV 8
          LST DG+V
Sbjct: 89 LSTEDGVV 96
>NP_001129674
          Length = 135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LSTADGLV 8
          LST++GL+
Sbjct: 88 LSTSEGLI 95
>NP_078986
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LSTADGLV 8
           LST++GL+
Sbjct: 174 LSTSEGLI 181
>NP_001273700
          Length = 2490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LSTADG 6
          LSTADG
Sbjct: 74 LSTADG 79
>NP_001183959
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 158 LSTADG 163
>NP_115593
          Length = 2603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 187 LSTADG 192
>NP_078944
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 158 LSTADG 163
>NP_942592
          Length = 2352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 187 LSTADG 192
>NP_060217
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 158 LSTADG 163
>NP_065741
          Length = 2617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 158 LSTADG 163
>XP_005259322
          Length = 151

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LSTADGLV 8
           LST++GL+
Sbjct: 104 LSTSEGLI 111
>XP_005258982
          Length = 201

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSTADGLV 8
           L TADG+V
Sbjct: 122 LLTADGMV 129
>XP_005266498
          Length = 531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSTADGLV 8
          LST DG+V
Sbjct: 89 LSTEDGVV 96
>XP_005265730
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 187 LSTADG 192
>XP_005265729
          Length = 1543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 187 LSTADG 192
>XP_005265728
          Length = 2351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 187 LSTADG 192
>XP_005265724
          Length = 2602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSTADG 6
           LSTADG
Sbjct: 187 LSTADG 192
>NP_001099040
          Length = 375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSTADGLV 8
           L TADG+V
Sbjct: 296 LLTADGMV 303
>NP_001116
          Length = 357

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LSTADGLVE 9
          L+TA+ LVE
Sbjct: 61 LATAEALVE 69
>NP_005583
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LSTADGLVE 9
          L T D LVE
Sbjct: 34 LETVDALVE 42
>NP_001159752
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LSTADGLVE 9
          L T D LVE
Sbjct: 34 LETVDALVE 42
>NP_001159753
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LSTADGLVE 9
          L T D LVE
Sbjct: 34 LETVDALVE 42
>NP_937784
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LSTADGLVE 9
           ++TA+GL++
Sbjct: 550 MATAEGLIK 558
>NP_061870
          Length = 87

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  TADGLVE 9
          T DGL+E
Sbjct: 31 TMDGLIE 37
>NP_114107
          Length = 87

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  TADGLVE 9
          T DGL+E
Sbjct: 31 TMDGLIE 37
>NP_653213
          Length = 2211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADGLVE 9
           ADGL+E
Sbjct: 403 ADGLIE 408
>XP_006722447
          Length = 1933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ADGLVE 9
           ADGL+E
Sbjct: 125 ADGLIE 130
>XP_005252053
          Length = 861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LSTADGLVE 9
          L T D LVE
Sbjct: 34 LETVDALVE 42
>XP_005252052
          Length = 871

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LSTADGLVE 9
          L T D LVE
Sbjct: 34 LETVDALVE 42
>XP_005252051
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LSTADGLVE 9
          L T D LVE
Sbjct: 34 LETVDALVE 42
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.127    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 474,378
Number of extensions: 449
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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