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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036462                                                              18    1482
XP_005269721                                                           18    1482
NP_001005191                                                           16    5631
NP_060102                                                              16    7355
NP_001009811                                                           15    9605
NP_055799                                                              15    9605
XP_005257233                                                           15    9605
XP_005257231                                                           15    9605

>NP_036462
          Length = 2073

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SSQKSSTA 9
           SSQKSSTA
Sbjct: 506 SSQKSSTA 513



>XP_005269721
          Length = 2073

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SSQKSSTA 9
           SSQKSSTA
Sbjct: 506 SSQKSSTA 513



>NP_001005191
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSQKSSTA 9
           SSQ SSTA
Sbjct: 267 SSQSSSTA 274



>NP_060102
          Length = 580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSQKSSTA 9
           +SSQ SST+
Sbjct: 196 ISSQNSSTS 204



>NP_001009811
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSQKSSTA 9
           L +QK+STA
Sbjct: 349 LRAQKASTA 357



>NP_055799
          Length = 1080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSQKSSTA 9
           L +QK+STA
Sbjct: 349 LRAQKASTA 357



>XP_005257233
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSQKSSTA 9
           L +QK+STA
Sbjct: 349 LRAQKASTA 357



>XP_005257231
          Length = 1080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSQKSSTA 9
           L +QK+STA
Sbjct: 349 LRAQKASTA 357



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.269   0.0813    0.160 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 236,101
Number of extensions: 200
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 19 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.5 bits)
S2: 28 (15.4 bits)