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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1482
NP_001075109                                                           18    1482
XP_006723719                                                           17    2528
XP_005260640                                                           16    7355
XP_005260639                                                           16    7355
NP_003601                                                              16    7355
NP_001015885                                                           16    7355
NP_078880                                                              15    9605
NP_001139547                                                           15    9605
NP_001181966                                                           15    9605
NP_001071174                                                           15    9605
XP_006715535                                                           15    9605
NP_001004692                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSSNKAISL 9
           LSSN+AISL
Sbjct: 840 LSSNEAISL 848



>NP_001075109
          Length = 4097

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSSNKAISL 9
           LSSN+AISL
Sbjct: 840 LSSNEAISL 848



>XP_006723719
          Length = 311

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   LSSNKAISL 9
           LSSNK+I+L
Sbjct: 273 LSSNKSITL 281



>XP_005260640
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSNKAISL 9
           LSSN+AI +
Sbjct: 153 LSSNQAIQI 161



>XP_005260639
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSNKAISL 9
           LSSN+AI +
Sbjct: 181 LSSNQAIQI 189



>NP_003601
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSNKAISL 9
           LSSN+AI +
Sbjct: 115 LSSNQAIQI 123



>NP_001015885
          Length = 368

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSSNKAISL 9
           LSSN+AI +
Sbjct: 115 LSSNQAIQI 123



>NP_078880
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SSNKAISL 9
          S+NKAI L
Sbjct: 3  SANKAIEL 10



>NP_001139547
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SSNKAISL 9
          S+NKAI L
Sbjct: 3  SANKAIEL 10



>NP_001181966
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSSNKAI 7
           LSSN+AI
Sbjct: 600 LSSNRAI 606



>NP_001071174
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSSNKAI 7
           LSSN+AI
Sbjct: 600 LSSNRAI 606



>XP_006715535
          Length = 946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSSNKAI 7
           LSSN+AI
Sbjct: 642 LSSNRAI 648



>NP_001004692
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSSNKAIS 8
          L+ NKAIS
Sbjct: 84 LTGNKAIS 91



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.112    0.243 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,923
Number of extensions: 361
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)