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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              17    2528
NP_996772                                                              17    4312
NP_997719                                                              16    5631
NP_940927                                                              15    9605
NP_006184                                                              15    9605
XP_006720560                                                           15    9605
XP_005254961                                                           15    9605
XP_005254960                                                           15    9605
XP_005254959                                                           15    9605
XP_005250454                                                           15    9605
NP_001004760                                                           15    9605

>NP_004295
          Length = 1620

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSRVLKVGS 9
           LSRVLK GS
Sbjct: 123 LSRVLKGGS 131



>NP_996772
          Length = 889

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LSRVLKVGS 9
           +SRVL+VG+
Sbjct: 874 MSRVLQVGA 882



>NP_997719
          Length = 291

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LSRVLKV 7
           LSRVLKV
Sbjct: 177 LSRVLKV 183



>NP_940927
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RVLKVGS 9
           RVLK GS
Sbjct: 258 RVLKTGS 264



>NP_006184
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LSRVLKVGS 9
          +SR+LKV +
Sbjct: 35 ISRILKVSN 43



>XP_006720560
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RVLKVGS 9
           RVLK GS
Sbjct: 258 RVLKTGS 264



>XP_005254961
          Length = 1342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RVLKVGS 9
           RVLK GS
Sbjct: 258 RVLKTGS 264



>XP_005254960
          Length = 1342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RVLKVGS 9
           RVLK GS
Sbjct: 258 RVLKTGS 264



>XP_005254959
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RVLKVGS 9
           RVLK GS
Sbjct: 258 RVLKTGS 264



>XP_005250454
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LSRVLKVGS 9
          +SR+LKV +
Sbjct: 43 ISRILKVSN 51



>NP_001004760
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SRVLKVG 8
           SR++K+G
Sbjct: 148 SRIIKIG 154



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.135    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 316,956
Number of extensions: 349
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)