Blast-Search of mutated query peptide against human proteome |
BLASTP 2.2.15 [Oct-15-2006] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= User_input (9 letters) Database: HUMAN_PROTEOME_FASTA 71,491 sequences; 45,948,801 total letters Searching..................................................doneScore E Sequences producing significant alignments: (bits) Value NP_004110 18 1935 NP_653271 15 9605 NP_001269353 15 9605>NP_004110 Length = 993 Score = 17.7 bits (34), Expect = 1935 Identities = 8/8 (100%), Positives = 8/8 (100%) Query: 2 SQSALVTI 9 SQSALVTI Sbjct: 337 SQSALVTI 344>NP_653271 Length = 1454 Score = 15.4 bits (28), Expect = 9605 Identities = 6/8 (75%), Positives = 7/8 (87%) Query: 1 LSQSALVT 8 +SQS LVT Sbjct: 1419 ISQSVLVT 1426>NP_001269353 Length = 963 Score = 15.4 bits (28), Expect = 9605 Identities = 6/8 (75%), Positives = 7/8 (87%) Query: 1 LSQSALVT 8 +SQS LVT Sbjct: 928 ISQSVLVT 935Database: HUMAN_PROTEOME_FASTA Posted date: May 26, 2014 10:46 AM Number of letters in database: 45,948,801 Number of sequences in database: 71,491 Lambda K H 0.315 0.122 0.263 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 71491 Number of Hits to DB: 197,032 Number of extensions: 118 Number of successful extensions: 3 Number of sequences better than 10000.0: 3 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 3 Length of query: 9 Length of database: 45,948,801 Length adjustment: 0 Effective length of query: 9 Effective length of database: 45,948,801 Effective search space: 413539209 Effective search space used: 413539209 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 28 (15.8 bits) S2: 28 (15.4 bits)
CANCERTOPE | Raghava's Group | IMTECH | CSIR | CRDD | GPSR Package |