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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001072993                                                           17    2528
NP_001265536                                                           16    7355
NP_001265537                                                           16    7355
NP_001265535                                                           16    7355
NP_001186                                                              16    7355
NP_001155177                                                           16    7355
NP_001035289                                                           15    9605
NP_001035273                                                           15    9605
NP_001035272                                                           15    9605
NP_001035271                                                           15    9605
NP_063948                                                              15    9605

>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LSNRITTAF 9
            LS+RITTAF
Sbjct: 1481 LSDRITTAF 1489



>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LSNRITTAF 9
            LS+RITTAF
Sbjct: 1276 LSDRITTAF 1284



>NP_001072993
          Length = 432

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  SNRITTAF 9
          SNRI TAF
Sbjct: 77 SNRIKTAF 84



>NP_001265536
          Length = 554

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NRITTAF 9
           NR+T+AF
Sbjct: 207 NRLTSAF 213



>NP_001265537
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NRITTAF 9
           NR+T+AF
Sbjct: 179 NRLTSAF 185



>NP_001265535
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NRITTAF 9
           NR+T+AF
Sbjct: 179 NRLTSAF 185



>NP_001186
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NRITTAF 9
           NR+T+AF
Sbjct: 318 NRLTSAF 324



>NP_001155177
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NRITTAF 9
           NR+T+AF
Sbjct: 193 NRLTSAF 199



>NP_001035289
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1 LSNRITTAF 9
         + NR  TAF
Sbjct: 1 MDNRFATAF 9



>NP_001035273
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1 LSNRITTAF 9
         + NR  TAF
Sbjct: 1 MDNRFATAF 9



>NP_001035272
          Length = 276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  LSNRITTAF 9
          + NR  TAF
Sbjct: 24 MDNRFATAF 32



>NP_001035271
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1 LSNRITTAF 9
         + NR  TAF
Sbjct: 1 MDNRFATAF 9



>NP_063948
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1 LSNRITTAF 9
         + NR  TAF
Sbjct: 1 MDNRFATAF 9



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.130    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 285,157
Number of extensions: 348
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)