Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005294                                                              17    3301
NP_001123308                                                           15    9605
NP_001123306                                                           15    9605
NP_001123312                                                           15    9605
NP_001123307                                                           15    9605
NP_001123316                                                           15    9605
NP_001123304                                                           15    9605
NP_001123305                                                           15    9605
NP_001123309                                                           15    9605
NP_001123299                                                           15    9605
NP_001123313                                                           15    9605
NP_000710                                                              15    9605
NP_001123314                                                           15    9605
NP_001123311                                                           15    9605
NP_001123310                                                           15    9605
NP_001123318                                                           15    9605
NP_001123301                                                           15    9605
NP_001123303                                                           15    9605
NP_001123315                                                           15    9605
NP_001123302                                                           15    9605
NP_001161095                                                           15    9605
NP_955630                                                              15    9605
NP_001161096                                                           15    9605
NP_001161097                                                           15    9605
NP_001185951                                                           15    9605
NP_055526                                                              15    9605
NP_057636                                                              15    9605
NP_001269700                                                           15    9605
XP_006721168                                                           15    9605
XP_005255778                                                           15    9605
XP_005255776                                                           15    9605
XP_006719080                                                           15    9605
XP_006719079                                                           15    9605
XP_006719078                                                           15    9605
XP_006712634                                                           15    9605
XP_005247077                                                           15    9605
XP_006712630                                                           15    9605
>NP_005294
          Length = 300

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LSMSMPLKA 9
          L++S+PLKA
Sbjct: 55 LTVSLPLKA 63
>NP_001123308
          Length = 2155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1561 VSMNMPLNS 1569
>NP_001123306
          Length = 2157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123312
          Length = 2138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123307
          Length = 2157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123316
          Length = 2135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1541 VSMNMPLNS 1549
>NP_001123304
          Length = 2158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1564 VSMNMPLNS 1572
>NP_001123305
          Length = 2157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123309
          Length = 2146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1533 VSMNMPLNS 1541
>NP_001123299
          Length = 2186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1592 VSMNMPLNS 1600
>NP_001123313
          Length = 2138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_000710
          Length = 2138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123314
          Length = 2138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123311
          Length = 2144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1531 VSMNMPLNS 1539
>NP_001123310
          Length = 2146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1533 VSMNMPLNS 1541
>NP_001123318
          Length = 2127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1533 VSMNMPLNS 1541
>NP_001123301
          Length = 2179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1566 VSMNMPLNS 1574
>NP_001123303
          Length = 2166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1572 VSMNMPLNS 1580
>NP_001123315
          Length = 2138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001123302
          Length = 2173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001161095
          Length = 2138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_955630
          Length = 2221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1592 VSMNMPLNS 1600
>NP_001161096
          Length = 2173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1544 VSMNMPLNS 1552
>NP_001161097
          Length = 2198

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1533 VSMNMPLNS 1541
>NP_001185951
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SMSMPLKA 9
           SMSMP+ A
Sbjct: 311 SMSMPVLA 318
>NP_055526
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SMSMPLKA 9
           SMSMP+ A
Sbjct: 311 SMSMPVLA 318
>NP_057636
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LSMSMPLKA 9
           LSM M LKA
Sbjct: 259 LSMEMILKA 267
>NP_001269700
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LSMSMPLKA 9
           LSM M LKA
Sbjct: 348 LSMEMILKA 356
>XP_006721168
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SMSMPLKA 9
           SMSMP+ A
Sbjct: 311 SMSMPVLA 318
>XP_005255778
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SMSMPLKA 9
           SMSMP+ A
Sbjct: 311 SMSMPVLA 318
>XP_005255776
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SMSMPLKA 9
           SMSMP+ A
Sbjct: 311 SMSMPVLA 318
>XP_006719080
          Length = 2168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1574 VSMNMPLNS 1582
>XP_006719079
          Length = 1459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSMSMPLKA 9
           +SM+MPL +
Sbjct: 846 VSMNMPLNS 854
>XP_006719078
          Length = 1905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LSMSMPLKA 9
            +SM+MPL +
Sbjct: 1292 VSMNMPLNS 1300
>XP_006712634
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  LSMSMPLKA 9
          LSM M LKA
Sbjct: 21 LSMEMILKA 29
>XP_005247077
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LSMSMPLKA 9
           LSM M LKA
Sbjct: 96  LSMEMILKA 104
>XP_006712630
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LSMSMPLKA 9
           LSM M LKA
Sbjct: 259 LSMEMILKA 267
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.120    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 411,163
Number of extensions: 291
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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