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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     869
XP_005266703                                                           19     869
NP_001129082                                                           17    4312
NP_001128669                                                           17    4312
XP_005265157                                                           17    4312
XP_005265156                                                           17    4312
XP_006713187                                                           17    4312
NP_835467                                                              17    4312
NP_067681                                                              16    7355
NP_001155000                                                           16    7355
NP_004360                                                              15    9605
NP_476508                                                              15    9605
NP_476507                                                              15    9605
NP_057571                                                              15    9605
NP_001135942                                                           15    9605
NP_001135940                                                           15    9605
XP_005267065                                                           15    9605
XP_005246123                                                           15    9605
XP_005246122                                                           15    9605
XP_003846701                                                           15    9605
NP_683765                                                              15    9605

>NP_443179
          Length = 2170

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SIGKSKVQ 9
            SIGKSKVQ
Sbjct: 2144 SIGKSKVQ 2151



>XP_005266703
          Length = 1501

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SIGKSKVQ 9
            SIGKSKVQ
Sbjct: 1475 SIGKSKVQ 1482



>NP_001129082
          Length = 226

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SIGKSKVQ 9
           SIG+SK+Q
Sbjct: 191 SIGRSKLQ 198



>NP_001128669
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSIGKSK 7
           LS+GKSK
Sbjct: 107 LSVGKSK 113



>XP_005265157
          Length = 577

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LSIGKSK 7
          LS+GKSK
Sbjct: 90 LSVGKSK 96



>XP_005265156
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSIGKSK 7
           LS+GKSK
Sbjct: 107 LSVGKSK 113



>XP_006713187
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSIGKSK 7
           LS+GKSK
Sbjct: 107 LSVGKSK 113



>NP_835467
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSIGKSK 7
           LS+GKSK
Sbjct: 107 LSVGKSK 113



>NP_067681
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1 LSIGKSKV 8
         + IGKSK+
Sbjct: 1 MGIGKSKI 8



>NP_001155000
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LSIGKSKVQ 9
           LS+GK+K++
Sbjct: 262 LSLGKNKLR 270



>NP_004360
          Length = 3177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LSIGKSKVQ 9
            L+IG SKV+
Sbjct: 1466 LNIGPSKVR 1474



>NP_476508
          Length = 2971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LSIGKSKVQ 9
            L+IG SKV+
Sbjct: 1260 LNIGPSKVR 1268



>NP_476507
          Length = 2570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LSIGKSKVQ 9
           L+IG SKV+
Sbjct: 859 LNIGPSKVR 867



>NP_057571
          Length = 240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSIGKSKVQ 9
           L IG++KV+
Sbjct: 152 LDIGRNKVE 160



>NP_001135942
          Length = 245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSIGKSKVQ 9
           L IG++KV+
Sbjct: 157 LDIGRNKVE 165



>NP_001135940
          Length = 240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSIGKSKVQ 9
           L IG++KV+
Sbjct: 152 LDIGRNKVE 160



>XP_005267065
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LSIGKSKVQ 9
           L IG++KV+
Sbjct: 182 LDIGRNKVE 190



>XP_005246123
          Length = 2770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LSIGKSKVQ 9
            L+IG SKV+
Sbjct: 1059 LNIGPSKVR 1067



>XP_005246122
          Length = 2977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LSIGKSKVQ 9
            L+IG SKV+
Sbjct: 1266 LNIGPSKVR 1274



>XP_003846701
          Length = 143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LSIGKSKVQ 9
           LS+GK + Q
Sbjct: 132 LSVGKKQAQ 140



>NP_683765
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SIGKSKVQ 9
           +IGK KVQ
Sbjct: 403 AIGKLKVQ 410



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.124    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 431,858
Number of extensions: 431
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)