Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1482
XP_005266703                                                           18    1482
NP_619542                                                              17    3301
NP_001276065                                                           17    3301
NP_004778                                                              17    3301
NP_001276064                                                           17    3301
XP_005274600                                                           17    3301
XP_005248271                                                           17    3301
XP_005248268                                                           17    3301
NP_938205                                                              16    5631
NP_001159430                                                           16    5631
NP_037412                                                              16    5631
NP_001035936                                                           16    5631
NP_001159431                                                           16    5631
NP_061000                                                              16    5631
NP_037413                                                              16    5631
NP_055052                                                              16    5631
XP_005260739                                                           16    5631
XP_005257568                                                           16    5631
XP_005257567                                                           16    5631
XP_005257565                                                           16    5631
XP_006722037                                                           16    5631
XP_005273918                                                           16    5631
XP_005265204                                                           16    5631
XP_006713221                                                           16    5631
XP_006713220                                                           16    5631
XP_006713219                                                           16    5631
XP_006713218                                                           16    5631
XP_006713217                                                           16    5631
XP_005265196                                                           16    5631
XP_005265195                                                           16    5631
XP_006713216                                                           16    5631
XP_006713215                                                           16    5631
XP_006713214                                                           16    5631
XP_006713213                                                           16    5631
XP_005265192                                                           16    5631
XP_005265191                                                           16    5631
XP_005265190                                                           16    5631
XP_006713212                                                           16    5631
XP_006713211                                                           16    5631
XP_006713210                                                           16    5631
XP_006713209                                                           16    5631
XP_006713208                                                           16    5631
XP_006713207                                                           16    5631
XP_006713206                                                           16    5631
XP_006713205                                                           16    5631
XP_006713204                                                           16    5631
XP_006713203                                                           16    5631
NP_059138                                                              16    7355
NP_689621                                                              16    7355
NP_001092741                                                           16    7355
XP_005248493                                                           16    7355
XP_005248492                                                           16    7355
XP_006714049                                                           16    7355
NP_001073881                                                           15    9605
NP_001239155                                                           15    9605
NP_006613                                                              15    9605
NP_055742                                                              15    9605
NP_001162118                                                           15    9605
NP_057278                                                              15    9605
NP_006569                                                              15    9605
NP_006392                                                              15    9605
NP_006296                                                              15    9605
NP_079216                                                              15    9605
XP_005274525                                                           15    9605
XP_006716465                                                           15    9605
XP_005273707                                                           15    9605
XP_006713622                                                           15    9605
XP_005247320                                                           15    9605
XP_005247319                                                           15    9605
XP_005247318                                                           15    9605
XP_005247315                                                           15    9605
XP_005247314                                                           15    9605
XP_005247313                                                           15    9605
XP_005247312                                                           15    9605
>NP_443179
          Length = 2170

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LSENRISTS 9
            LSENRIS S
Sbjct: 1462 LSENRISRS 1470
>XP_005266703
          Length = 1501

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSENRISTS 9
           LSENRIS S
Sbjct: 793 LSENRISRS 801
>NP_619542
          Length = 1041

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           LSENRIS
Sbjct: 425 LSENRIS 431
>NP_001276065
          Length = 1521

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           LS NRIST
Sbjct: 776 LSNNRIST 783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSENRIST 8
          L EN+IST
Sbjct: 86 LMENKIST 93
>NP_004778
          Length = 1529

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           LS NRIST
Sbjct: 784 LSNNRIST 791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSENRIST 8
          L EN+IST
Sbjct: 86 LMENKIST 93
>NP_001276064
          Length = 1525

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           LS NRIST
Sbjct: 780 LSNNRIST 787

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSENRIST 8
          L EN+IST
Sbjct: 86 LMENKIST 93
>XP_005274600
          Length = 1059

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           LSENRIS
Sbjct: 443 LSENRIS 449
>XP_005248271
          Length = 1404

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           LS NRIST
Sbjct: 659 LSNNRIST 666
>XP_005248268
          Length = 1533

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           LS NRIST
Sbjct: 788 LSNNRIST 795

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSENRIST 8
          L EN+IST
Sbjct: 86 LMENKIST 93
>NP_938205
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L +NRIST
Sbjct: 182 LDDNRIST 189
>NP_001159430
          Length = 416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>NP_037412
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L +NRIST
Sbjct: 205 LDDNRIST 212
>NP_001035936
          Length = 403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>NP_001159431
          Length = 407

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>NP_061000
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>NP_037413
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L +NRIST
Sbjct: 182 LDDNRIST 189
>NP_055052
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SENRISTS 9
           +EN+ISTS
Sbjct: 239 NENQISTS 246
>XP_005260739
          Length = 649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L +NRIST
Sbjct: 182 LDDNRIST 189
>XP_005257568
          Length = 224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>XP_005257567
          Length = 403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>XP_005257565
          Length = 416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>XP_006722037
          Length = 416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           +SENRIS
Sbjct: 120 ISENRIS 126
>XP_005273918
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L +NRIST
Sbjct: 205 LDDNRIST 212
>XP_005265204
          Length = 2256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1260 LGENKIST 1267
>XP_006713221
          Length = 2268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_006713220
          Length = 2274

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1278 LGENKIST 1285
>XP_006713219
          Length = 2280

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_006713218
          Length = 2282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1286 LGENKIST 1293
>XP_006713217
          Length = 2283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_005265196
          Length = 2297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1301 LGENKIST 1308
>XP_005265195
          Length = 2297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1301 LGENKIST 1308
>XP_006713216
          Length = 2300

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1304 LGENKIST 1311
>XP_006713215
          Length = 2309

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1313 LGENKIST 1320
>XP_006713214
          Length = 2311

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1315 LGENKIST 1322
>XP_006713213
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_005265192
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1318 LGENKIST 1325
>XP_005265191
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1318 LGENKIST 1325
>XP_005265190
          Length = 2314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1318 LGENKIST 1325
>XP_006713212
          Length = 2320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_006713211
          Length = 2323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1327 LGENKIST 1334
>XP_006713210
          Length = 2326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1330 LGENKIST 1337
>XP_006713209
          Length = 2326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1330 LGENKIST 1337
>XP_006713208
          Length = 2335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_006713207
          Length = 2335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1339 LGENKIST 1346
>XP_006713206
          Length = 2337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_006713205
          Length = 2340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1344 LGENKIST 1351
>XP_006713204
          Length = 2351

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>XP_006713203
          Length = 2352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LSENRIST 8
            L EN+IST
Sbjct: 1356 LGENKIST 1363
>NP_059138
          Length = 1032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LSENRIS 7
           LS+NRIS
Sbjct: 422 LSDNRIS 428
>NP_689621
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SENRISTS 9
           S+NRIS+S
Sbjct: 473 SKNRISSS 480
>NP_001092741
          Length = 575

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SENRISTS 9
           S+NRIS+S
Sbjct: 498 SKNRISSS 505
>XP_005248493
          Length = 547

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SENRISTS 9
           S+NRIS+S
Sbjct: 470 SKNRISSS 477
>XP_005248492
          Length = 548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SENRISTS 9
           S+NRIS+S
Sbjct: 471 SKNRISSS 478
>XP_006714049
          Length = 1509

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSENRIST 8
          L EN+IST
Sbjct: 86 LMENKIST 93
>NP_001073881
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>NP_001239155
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LSENRISTS 9
           ++E+RIST+
Sbjct: 632 INEDRISTT 640
>NP_006613
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LSENRISTS 9
           ++E+RIST+
Sbjct: 646 INEDRISTT 654
>NP_055742
          Length = 1034

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L E+RIST
Sbjct: 924 LGEHRIST 931
>NP_001162118
          Length = 1004

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L E+RIST
Sbjct: 894 LGEHRIST 901
>NP_057278
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ENRIST 8
           ENR+ST
Sbjct: 367 ENRVST 372
>NP_006569
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ENRIST 8
           ENR+ST
Sbjct: 325 ENRVST 330
>NP_006392
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LSENRI 6
          LSENRI
Sbjct: 71 LSENRI 76
>NP_006296
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LSENRIS 7
          LS+NR+S
Sbjct: 71 LSDNRVS 77
>NP_079216
          Length = 1870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SENRIS 7
            SENRIS
Sbjct: 1725 SENRIS 1730
>XP_005274525
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSENRIST 8
           L E+RIST
Sbjct: 875 LGEHRIST 882
>XP_006716465
          Length = 1826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SENRIS 7
            SENRIS
Sbjct: 1725 SENRIS 1730
>XP_005273707
          Length = 1847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SENRIS 7
            SENRIS
Sbjct: 1725 SENRIS 1730
>XP_006713622
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>XP_005247320
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>XP_005247319
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>XP_005247318
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>XP_005247315
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>XP_005247314
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 429 LSENRI 434
>XP_005247313
          Length = 1196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 430 LSENRI 435
>XP_005247312
          Length = 1231

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LSENRI 6
           LSENRI
Sbjct: 465 LSENRI 470
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.107    0.237 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,151
Number of extensions: 516
Number of successful extensions: 79
Number of sequences better than 10000.0: 75
Number of HSP's gapped: 79
Number of HSP's successfully gapped: 79
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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