Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              18    1482
XP_006713721                                                           18    1482
XP_005249373                                                           17    3301
NP_847894                                                              15    9605
NP_060888                                                              15    9605
NP_001268232                                                           15    9605
NP_006406                                                              15    9605
NP_001035518                                                           15    9605
NP_892006                                                              15    9605
NP_149062                                                              15    9605
NP_057358                                                              15    9605
NP_001273047                                                           15    9605
NP_001273048                                                           15    9605
NP_004271                                                              15    9605
NP_004454                                                              15    9605
NP_060697                                                              15    9605
XP_005267912                                                           15    9605
XP_005267911                                                           15    9605
XP_005267909                                                           15    9605
XP_005267908                                                           15    9605
XP_005274213                                                           15    9605
XP_005274211                                                           15    9605
XP_006715488                                                           15    9605
XP_006715487                                                           15    9605
XP_006715486                                                           15    9605
XP_006715485                                                           15    9605
XP_006715484                                                           15    9605
XP_006715483                                                           15    9605
XP_006715482                                                           15    9605
XP_006715481                                                           15    9605
XP_006715480                                                           15    9605
XP_006715479                                                           15    9605
XP_006715478                                                           15    9605
XP_006715477                                                           15    9605
XP_006715476                                                           15    9605
XP_006715475                                                           15    9605
XP_006715474                                                           15    9605
XP_006715473                                                           15    9605
XP_006715472                                                           15    9605
XP_006715471                                                           15    9605
XP_006715470                                                           15    9605
XP_006714316                                                           15    9605
>NP_006209
          Length = 1068

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSEITVQEK 9
           LSEIT QEK
Sbjct: 540 LSEITEQEK 548
>XP_006713721
          Length = 1068

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSEITVQEK 9
           LSEIT QEK
Sbjct: 540 LSEITEQEK 548
>XP_005249373
          Length = 7723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SEITVQEK 9
           SEIT QEK
Sbjct: 166 SEITFQEK 173
>NP_847894
          Length = 143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SEITVQEK 9
          S++TV+EK
Sbjct: 50 SDLTVKEK 57
>NP_060888
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SEITVQEK 9
           SE+T+Q++
Sbjct: 456 SEVTIQQE 463
>NP_001268232
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SEITVQEK 9
          S++TV+EK
Sbjct: 50 SDLTVKEK 57
>NP_006406
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  SEITVQEK 9
          S++TV+EK
Sbjct: 50 SDLTVKEK 57
>NP_001035518
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SEITVQEK 9
           SE+T+Q++
Sbjct: 424 SEVTIQQE 431
>NP_892006
          Length = 8797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4521 MKEVTEQEK 4529
>NP_149062
          Length = 8749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4450 MKEVTEQEK 4458
>NP_057358
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSEITVQE 8
           L E+TV+E
Sbjct: 139 LDEVTVEE 146
>NP_001273047
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSEITVQE 8
          L EITVQ+
Sbjct: 9  LMEITVQQ 16
>NP_001273048
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSEITVQE 8
          L EITVQ+
Sbjct: 9  LMEITVQQ 16
>NP_004271
          Length = 174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EITVQEK 9
           ++TVQEK
Sbjct: 130 DLTVQEK 136
>NP_004454
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EITVQEK 9
           E+TVQ+K
Sbjct: 367 ELTVQQK 373
>NP_060697
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSEITVQE 8
           LSE+ +QE
Sbjct: 787 LSEVKIQE 794
>XP_005267912
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SEITVQEK 9
           SE+T+Q++
Sbjct: 393 SEVTIQQE 400
>XP_005267911
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SEITVQEK 9
           SE+T+Q++
Sbjct: 393 SEVTIQQE 400
>XP_005267909
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SEITVQEK 9
           SE+T+Q++
Sbjct: 425 SEVTIQQE 432
>XP_005267908
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   SEITVQEK 9
           SE+T+Q++
Sbjct: 457 SEVTIQQE 464
>XP_005274213
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSEITVQE 8
          L EITVQ+
Sbjct: 9  LMEITVQQ 16
>XP_005274211
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LSEITVQE 8
          L EITVQ+
Sbjct: 9  LMEITVQQ 16
>XP_006715488
          Length = 8721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715487
          Length = 8744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715486
          Length = 8758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715485
          Length = 8793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4475 MKEVTEQEK 4483
>XP_006715484
          Length = 8794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4476 MKEVTEQEK 4484
>XP_006715483
          Length = 8795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715482
          Length = 8797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4521 MKEVTEQEK 4529
>XP_006715481
          Length = 8799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4481 MKEVTEQEK 4489
>XP_006715480
          Length = 8799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715479
          Length = 8804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715478
          Length = 8809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715477
          Length = 8822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4504 MKEVTEQEK 4512
>XP_006715476
          Length = 8823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715475
          Length = 8827

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715474
          Length = 8829

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4511 MKEVTEQEK 4519
>XP_006715473
          Length = 8832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715472
          Length = 8839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4521 MKEVTEQEK 4529
>XP_006715471
          Length = 8842

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006715470
          Length = 8846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LSEITVQEK 9
            + E+T QEK
Sbjct: 4528 MKEVTEQEK 4536
>XP_006714316
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSEITVQE 8
           LSE+ +QE
Sbjct: 712 LSEVKIQE 719
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.117    0.257 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 468,722
Number of extensions: 847
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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