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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001263407                                                           19     665
NP_004959                                                              19     665
NP_001129492                                                           19     665
NP_071682                                                              19     665
XP_006715784                                                           19     665
XP_006715783                                                           19     665
XP_005249793                                                           19     665
XP_005249792                                                           19     665
XP_006715782                                                           19     665
XP_006715781                                                           19     665
XP_006715780                                                           19     665
NP_005179                                                              18    1935
NP_001116836                                                           18    1935
NP_001120843                                                           17    4312
NP_061189                                                              17    4312
NP_783864                                                              16    5631
NP_001019554                                                           16    5631
NP_003696                                                              16    5631
NP_569157                                                              16    5631
NP_009058                                                              16    5631
NP_055718                                                              16    5631
XP_006719740                                                           16    5631
XP_006719739                                                           16    5631
XP_006719738                                                           16    5631
XP_005269272                                                           16    5631
XP_005262701                                                           16    5631
XP_005246980                                                           16    5631
NP_001028255                                                           16    7355
NP_071412                                                              16    7355
XP_005270086                                                           16    7355
XP_005270085                                                           16    7355
NP_001243690                                                           15    9605
NP_001243689                                                           15    9605
NP_000859                                                              15    9605
NP_689778                                                              15    9605
NP_775871                                                              15    9605

>NP_001263407
          Length = 482

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 375 LSEIFNASS 383



>NP_004959
          Length = 483

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 376 LSEIFNASS 384



>NP_001129492
          Length = 483

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 376 LSEIFNASS 384



>NP_071682
          Length = 483

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 376 LSEIFNASS 384



>XP_006715784
          Length = 274

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 167 LSEIFNASS 175



>XP_006715783
          Length = 482

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 375 LSEIFNASS 383



>XP_005249793
          Length = 482

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 375 LSEIFNASS 383



>XP_005249792
          Length = 482

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 375 LSEIFNASS 383



>XP_006715782
          Length = 483

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 376 LSEIFNASS 384



>XP_006715781
          Length = 483

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 376 LSEIFNASS 384



>XP_006715780
          Length = 483

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LSDIFNASS 9
           LS+IFNASS
Sbjct: 376 LSEIFNASS 384



>NP_005179
          Length = 906

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSDIFNASS 9
           LSDI NASS
Sbjct: 790 LSDISNASS 798



>NP_001116836
          Length = 366

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   DIFNASS 9
           DIFNASS
Sbjct: 348 DIFNASS 354



>NP_001120843
          Length = 408

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LSDIFNASS 9
          ++DIFNA S
Sbjct: 13 VADIFNAPS 21



>NP_061189
          Length = 454

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LSDIFNASS 9
          ++DIFNA S
Sbjct: 13 VADIFNAPS 21



>NP_783864
          Length = 1044

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSDIFNAS 8
           LSDI+N S
Sbjct: 697 LSDIYNRS 704



>NP_001019554
          Length = 2385

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LSDIFN 6
            LSDIFN
Sbjct: 2134 LSDIFN 2139



>NP_003696
          Length = 678

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LSDIFNAS 8
           L+D++NAS
Sbjct: 274 LADLYNAS 281



>NP_569157
          Length = 295

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  SDIFNAS 8
          SDIFN S
Sbjct: 52 SDIFNTS 58



>NP_009058
          Length = 827

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   DIFNASS 9
           D+FNA+S
Sbjct: 751 DVFNANS 757



>NP_055718
          Length = 2363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LSDIFN 6
            LSDIFN
Sbjct: 2112 LSDIFN 2117



>XP_006719740
          Length = 1863

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LSDIFN 6
            LSDIFN
Sbjct: 1612 LSDIFN 1617



>XP_006719739
          Length = 2306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LSDIFN 6
            LSDIFN
Sbjct: 2055 LSDIFN 2060



>XP_006719738
          Length = 2340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LSDIFN 6
            LSDIFN
Sbjct: 2089 LSDIFN 2094



>XP_005269272
          Length = 2356

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    LSDIFN 6
            LSDIFN
Sbjct: 2105 LSDIFN 2110



>XP_005262701
          Length = 1035

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSDIFNAS 8
           LSDI+N S
Sbjct: 688 LSDIYNRS 695



>XP_005246980
          Length = 661

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LSDIFNAS 8
           L+D++NAS
Sbjct: 257 LADLYNAS 264



>NP_001028255
          Length = 280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSDIFNAS 8
           LSD++N S
Sbjct: 165 LSDVYNRS 172



>NP_071412
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSDIFNAS 8
           LSD++N S
Sbjct: 165 LSDVYNRS 172



>XP_005270086
          Length = 280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSDIFNAS 8
           LSD++N S
Sbjct: 165 LSDVYNRS 172



>XP_005270085
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LSDIFNAS 8
           LSD++N S
Sbjct: 165 LSDVYNRS 172



>NP_001243690
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LSDIFNAS 8
          ++DIFN S
Sbjct: 34 VTDIFNTS 41



>NP_001243689
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LSDIFNAS 8
          ++DIFN S
Sbjct: 34 VTDIFNTS 41



>NP_000859
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LSDIFNAS 8
          ++DIFN S
Sbjct: 34 VTDIFNTS 41



>NP_689778
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SDIFNA 7
           SD+FNA
Sbjct: 148 SDLFNA 153



>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   IFNASS 9
           IFNASS
Sbjct: 908 IFNASS 913



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.122    0.300 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 397,017
Number of extensions: 502
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)