Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002115                                                              17    3301
NP_001234                                                              17    3301
NP_001268359                                                           17    3301
NP_056007                                                              17    3301
NP_055055                                                              17    3301
XP_006711112                                                           17    3301
NP_001138727                                                           17    4312
NP_001093326                                                           17    4312
NP_001093327                                                           17    4312
NP_003630                                                              17    4312
XP_005274821                                                           17    4312
XP_005274820                                                           17    4312
XP_006724916                                                           17    4312
XP_005274817                                                           17    4312
NP_997468                                                              16    5631
NP_001276054                                                           16    5631
NP_002116                                                              16    7355
NP_699171                                                              16    7355
NP_068818                                                              16    7355
XP_005252364                                                           16    7355
XP_005270503                                                           16    7355
XP_005270502                                                           16    7355
XP_006710306                                                           16    7355
XP_006710237                                                           16    7355
NP_001230894                                                           16    7355
NP_001035987                                                           15    9605
NP_001177396                                                           15    9605
XP_006723282                                                           15    9605
XP_006720939                                                           15    9605
XP_006720938                                                           15    9605
XP_005263896                                                           15    9605
XP_005263895                                                           15    9605
XP_005263893                                                           15    9605
NP_036599                                                              15    9605
>NP_002115
          Length = 266

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          LS+PLALS
Sbjct: 22 LSSPLALS 29
>NP_001234
          Length = 595

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SAPLALSS 9
           SAP+ALSS
Sbjct: 369 SAPVALSS 376
>NP_001268359
          Length = 483

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SAPLALSS 9
           SAP+ALSS
Sbjct: 258 SAPVALSS 265
>NP_056007
          Length = 1216

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    SAPLALSS 9
            SAPL+LSS
Sbjct: 1181 SAPLSLSS 1188
>NP_055055
          Length = 676

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   LSAPLALS 8
           +SAPLALS
Sbjct: 297 VSAPLALS 304
>XP_006711112
          Length = 545

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SAPLALSS 9
           SAP+ALSS
Sbjct: 320 SAPVALSS 327
>NP_001138727
          Length = 320

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 276 LSSPLALPS 284
>NP_001093326
          Length = 487

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 443 LSSPLALPS 451
>NP_001093327
          Length = 419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 375 LSSPLALPS 383
>NP_003630
          Length = 419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 375 LSSPLALPS 383
>XP_005274821
          Length = 418

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 374 LSSPLALPS 382
>XP_005274820
          Length = 419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 375 LSSPLALPS 383
>XP_006724916
          Length = 461

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 417 LSSPLALPS 425
>XP_005274817
          Length = 486

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LS+PLAL S
Sbjct: 442 LSSPLALPS 450
>NP_997468
          Length = 515

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LSAPLALSS 9
           L APL LSS
Sbjct: 443 LEAPLMLSS 451
>NP_001276054
          Length = 515

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LSAPLALSS 9
           L APL LSS
Sbjct: 443 LEAPLMLSS 451
>NP_002116
          Length = 266

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          LS+PLAL+
Sbjct: 22 LSSPLALA 29
>NP_699171
          Length = 722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LSAPL+ SS
Sbjct: 470 LSAPLSPSS 478
>NP_068818
          Length = 266

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          LS+PLAL+
Sbjct: 22 LSSPLALA 29
>XP_005252364
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  LSAPLALSS 9
          L+AP ALSS
Sbjct: 67 LAAPPALSS 75
>XP_005270503
          Length = 754

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LSAPL+ SS
Sbjct: 502 LSAPLSPSS 510
>XP_005270502
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LSAPLALSS 9
           LSAPL+ SS
Sbjct: 502 LSAPLSPSS 510
>XP_006710306
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          LS+PLAL+
Sbjct: 22 LSSPLALA 29
>XP_006710237
          Length = 274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          LS+PLAL+
Sbjct: 22 LSSPLALA 29
>NP_001230894
          Length = 266

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          LS+PLAL+
Sbjct: 22 LSSPLALA 29
>NP_001035987
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   APLALSS 9
           APLALS+
Sbjct: 230 APLALST 236
>NP_001177396
          Length = 1346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   APLALSS 9
           +PLALSS
Sbjct: 604 SPLALSS 610
>XP_006723282
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   APLALSS 9
           APLALS+
Sbjct: 230 APLALST 236
>XP_006720939
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSAPLALSS 9
           LS P+ LSS
Sbjct: 156 LSVPVTLSS 164
>XP_006720938
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSAPLALSS 9
           LS P+ LSS
Sbjct: 156 LSVPVTLSS 164
>XP_005263896
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          +S+P+ALS
Sbjct: 26 ISSPMALS 33
>XP_005263895
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          +S+P+ALS
Sbjct: 26 ISSPMALS 33
>XP_005263893
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LSAPLALS 8
          +S+P+ALS
Sbjct: 26 ISSPMALS 33
>NP_036599
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LSAPLALSS 9
           LS P+ LSS
Sbjct: 131 LSVPVTLSS 139
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.115    0.274 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 546,807
Number of extensions: 906
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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