Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              16    5631
NP_001137254                                                           16    5631
NP_057678                                                              16    5631
NP_001136013                                                           16    7355
NP_057032                                                              16    7355
XP_005260183                                                           16    7355
XP_006715167                                                           16    7355
XP_005249215                                                           16    7355
XP_006715166                                                           16    7355
XP_006715165                                                           16    7355
XP_005249214                                                           16    7355
XP_005249213                                                           16    7355
NP_001177693                                                           16    7355
NP_149014                                                              16    7355
NP_038478                                                              15    9605
NP_001276904                                                           15    9605
NP_659452                                                              15    9605
NP_001091985                                                           15    9605
XP_006712537                                                           15    9605
XP_006712536                                                           15    9605
XP_005246557                                                           15    9605
XP_006712535                                                           15    9605
XP_006712534                                                           15    9605
XP_006712533                                                           15    9605
XP_006712532                                                           15    9605
XP_006712531                                                           15    9605
XP_006712530                                                           15    9605
XP_006712529                                                           15    9605
XP_006712528                                                           15    9605
XP_006712527                                                           15    9605
XP_005246549                                                           15    9605
XP_006712526                                                           15    9605
XP_006712525                                                           15    9605
XP_005246546                                                           15    9605
XP_006712524                                                           15    9605
XP_005246545                                                           15    9605
>NP_004295
          Length = 1620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LSAAICASQ 9
           LSAAI ASQ
Sbjct: 204 LSAAIRASQ 212
>NP_001137254
          Length = 146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LSAAICASQ 9
          LSAA+C ++
Sbjct: 18 LSAAVCRAE 26
>NP_057678
          Length = 201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LSAAICASQ 9
          LSAA+C ++
Sbjct: 18 LSAAVCRAE 26
>NP_001136013
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 200 VSAAICMS 207
>NP_057032
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 200 VSAAICMS 207
>XP_005260183
          Length = 668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAAICASQ 9
           SAA+C S+
Sbjct: 388 SAAVCLSE 395
>XP_006715167
          Length = 285

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 202 VSAAICMS 209
>XP_005249215
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 168 VSAAICMS 175
>XP_006715166
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 202 VSAAICMS 209
>XP_006715165
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 200 VSAAICMS 207
>XP_005249214
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 200 VSAAICMS 207
>XP_005249213
          Length = 403

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LSAAICAS 8
           +SAAIC S
Sbjct: 202 VSAAICMS 209
>NP_001177693
          Length = 176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AICASQ 9
          A+CASQ
Sbjct: 6  AVCASQ 11
>NP_149014
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAAICASQ 9
           SAA+C S+
Sbjct: 455 SAAVCLSE 462
>NP_038478
          Length = 2168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1143 LSAAVC 1148
>NP_001276904
          Length = 2132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1107 LSAAVC 1112
>NP_659452
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   LSAAICA 7
           LSA +CA
Sbjct: 159 LSAVVCA 165
>NP_001091985
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   LSAAICA 7
           LSA +CA
Sbjct: 159 LSAVVCA 165
>XP_006712537
          Length = 1952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1154 LSAAVC 1159
>XP_006712536
          Length = 2082

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1022 LSAAVC 1027
>XP_005246557
          Length = 2095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1035 LSAAVC 1040
>XP_006712535
          Length = 2095

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1035 LSAAVC 1040
>XP_006712534
          Length = 2114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1054 LSAAVC 1059
>XP_006712533
          Length = 2116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1056 LSAAVC 1061
>XP_006712532
          Length = 2141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1116 LSAAVC 1121
>XP_006712531
          Length = 2169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1109 LSAAVC 1114
>XP_006712530
          Length = 2176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1116 LSAAVC 1121
>XP_006712529
          Length = 2179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1154 LSAAVC 1159
>XP_006712528
          Length = 2180

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1120 LSAAVC 1125
>XP_006712527
          Length = 2203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1143 LSAAVC 1148
>XP_005246549
          Length = 2212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1152 LSAAVC 1157
>XP_006712526
          Length = 2212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1152 LSAAVC 1157
>XP_006712525
          Length = 2212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1152 LSAAVC 1157
>XP_005246546
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1154 LSAAVC 1159
>XP_006712524
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1154 LSAAVC 1159
>XP_005246545
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    LSAAIC 6
            LSAA+C
Sbjct: 1154 LSAAVC 1159
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.114    0.311 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 528,358
Number of extensions: 470
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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