Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              19     869
NP_001207417                                                           16    5631
XP_006721183                                                           16    5631
NP_001248                                                              16    5631
NP_001207419                                                           16    5631
NP_001207418                                                           16    5631
NP_001137472                                                           16    7355
NP_001073936                                                           16    7355
NP_001177739                                                           16    7355
NP_060358                                                              16    7355
XP_006721119                                                           16    7355
XP_006721118                                                           16    7355
NP_597704                                                              16    7355
NP_116206                                                              15    9605
NP_085046                                                              15    9605
NP_001103444                                                           15    9605
NP_001159681                                                           15    9605
NP_001159680                                                           15    9605
NP_009217                                                              15    9605
XP_005268428                                                           15    9605
XP_005268427                                                           15    9605
XP_005268426                                                           15    9605
XP_006714818                                                           15    9605
>NP_004295
          Length = 1620

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRTSTIMID 9
            LRTSTIM D
Sbjct: 1083 LRTSTIMTD 1091
>NP_001207417
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSTIMID 9
           T+TIMID
Sbjct: 395 TATIMID 401
>XP_006721183
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSTIMID 9
           T+TIMID
Sbjct: 348 TATIMID 354
>NP_001248
          Length = 713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSTIMID 9
           T+TIMID
Sbjct: 348 TATIMID 354
>NP_001207419
          Length = 459

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSTIMID 9
           T+TIMID
Sbjct: 94  TATIMID 100
>NP_001207418
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TSTIMID 9
           T+TIMID
Sbjct: 309 TATIMID 315
>NP_001137472
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LRTSTIMI 8
           LRTSTI +
Sbjct: 333 LRTSTIKV 340
>NP_001073936
          Length = 1848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RTSTIMI 8
           RT+TIMI
Sbjct: 765 RTATIMI 771
>NP_001177739
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LRTSTIMI 8
           LRTSTI +
Sbjct: 333 LRTSTIKV 340
>NP_060358
          Length = 579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LRTSTIMI 8
           +RT T+MI
Sbjct: 136 MRTGTVMI 143
>XP_006721119
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LRTSTIMI 8
           +RT T+MI
Sbjct: 136 MRTGTVMI 143
>XP_006721118
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LRTSTIMI 8
           +RT T+MI
Sbjct: 136 MRTGTVMI 143
>NP_597704
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LRTSTIMI 8
           LRTSTI +
Sbjct: 172 LRTSTIKV 179
>NP_116206
          Length = 728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           LRT TI+I+
Sbjct: 527 LRTITILIN 535
>NP_085046
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           LRT TI+I+
Sbjct: 741 LRTITILIN 749
>NP_001103444
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 150 VRCSTLLID 158
>NP_001159681
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 394 VRCSTLLID 402
>NP_001159680
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 394 VRCSTLLID 402
>NP_009217
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 150 VRCSTLLID 158
>XP_005268428
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 150 VRCSTLLID 158
>XP_005268427
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 150 VRCSTLLID 158
>XP_005268426
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 150 VRCSTLLID 158
>XP_006714818
          Length = 1147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRTSTIMID 9
           +R ST++ID
Sbjct: 394 VRCSTLLID 402
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.137    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 258,350
Number of extensions: 303
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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