Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              20     299
NP_005913                                                              20     299
XP_005267046                                                           20     299
XP_005267045                                                           20     299
NP_079001                                                              17    4312
XP_006718974                                                           17    4312
XP_006718973                                                           17    4312
XP_006718972                                                           17    4312
XP_005274350                                                           17    4312
XP_005274349                                                           17    4312
NP_006493                                                              16    5631
NP_004963                                                              16    5631
XP_005251512                                                           16    5631
XP_006715181                                                           16    5631
XP_005249243                                                           16    5631
NP_031392                                                              16    7355
NP_001007268                                                           16    7355
NP_001182570                                                           16    7355
NP_570129                                                              16    7355
XP_005246450                                                           16    7355
XP_005246449                                                           16    7355
XP_006712321                                                           16    7355
NP_659001                                                              15    9605
NP_001154178                                                           15    9605
XP_005266635                                                           15    9605
XP_006719947                                                           15    9605
XP_006719946                                                           15    9605
XP_006719945                                                           15    9605
>NP_006715
          Length = 1558

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRSMQVDNI 9
           LRSMQVDN+
Sbjct: 937 LRSMQVDNL 945
>NP_005913
          Length = 1608

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRSMQVDNI 9
           LRSMQVDN+
Sbjct: 937 LRSMQVDNL 945
>XP_005267046
          Length = 1554

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRSMQVDNI 9
           LRSMQVDN+
Sbjct: 937 LRSMQVDNL 945
>XP_005267045
          Length = 1604

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRSMQVDNI 9
           LRSMQVDN+
Sbjct: 937 LRSMQVDNL 945
>NP_079001
          Length = 544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RSMQVDN 8
           R+MQ+DN
Sbjct: 447 RTMQIDN 453
>XP_006718974
          Length = 521

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RSMQVDN 8
           R+MQ+DN
Sbjct: 424 RTMQIDN 430
>XP_006718973
          Length = 521

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RSMQVDN 8
           R+MQ+DN
Sbjct: 424 RTMQIDN 430
>XP_006718972
          Length = 537

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RSMQVDN 8
           R+MQ+DN
Sbjct: 447 RTMQIDN 453
>XP_005274350
          Length = 544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RSMQVDN 8
           R+MQ+DN
Sbjct: 447 RTMQIDN 453
>XP_005274349
          Length = 544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RSMQVDN 8
           R+MQ+DN
Sbjct: 447 RTMQIDN 453
>NP_006493
          Length = 713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRSMQVDNI 9
           L S+Q+DN+
Sbjct: 175 LDSLQIDNL 183
>NP_004963
          Length = 1132

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRSMQVDNI 9
           L+S+Q DNI
Sbjct: 902 LKSLQHDNI 910
>XP_005251512
          Length = 1132

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRSMQVDNI 9
           L+S+Q DNI
Sbjct: 902 LKSLQHDNI 910
>XP_006715181
          Length = 561

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LRSMQVDNI 9
          L S+Q+DN+
Sbjct: 23 LDSLQIDNL 31
>XP_005249243
          Length = 651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRSMQVDNI 9
           L S+Q+DN+
Sbjct: 113 LDSLQIDNL 121
>NP_031392
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LRSMQVDN 8
           LRS Q DN
Sbjct: 403 LRSCQADN 410
>NP_001007268
          Length = 1324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LRSMQVDN 8
           LRS Q DN
Sbjct: 403 LRSCQADN 410
>NP_001182570
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LRSMQVDN 8
           LRS Q DN
Sbjct: 403 LRSCQADN 410
>NP_570129
          Length = 1306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SMQVDNI 9
           S+QVDN+
Sbjct: 887 SLQVDNL 893
>XP_005246450
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LRSMQVDN 8
           LRS Q DN
Sbjct: 403 LRSCQADN 410
>XP_005246449
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   LRSMQVDN 8
           LRS Q DN
Sbjct: 403 LRSCQADN 410
>XP_006712321
          Length = 1307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SMQVDNI 9
           S+QVDN+
Sbjct: 888 SLQVDNL 894
>NP_659001
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRSMQVDNI 9
           +RS  +DNI
Sbjct: 230 IRSQDLDNI 238
>NP_001154178
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRSMQVDNI 9
           +RS  +DNI
Sbjct: 208 IRSQDLDNI 216
>XP_005266635
          Length = 665

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRSMQVDNI 9
           +RS  +DNI
Sbjct: 207 IRSQDLDNI 215
>XP_006719947
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRSMQVDNI 9
           +RS  +DNI
Sbjct: 208 IRSQDLDNI 216
>XP_006719946
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRSMQVDNI 9
           +RS  +DNI
Sbjct: 230 IRSQDLDNI 238
>XP_006719945
          Length = 668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRSMQVDNI 9
           +RS  +DNI
Sbjct: 230 IRSQDLDNI 238
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.135    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 359,219
Number of extensions: 377
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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