Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_598328                                                              17    3301
NP_001129246                                                           17    3301
NP_008881                                                              17    3301
XP_005276380                                                           17    3301
XP_005251180                                                           17    3301
XP_006713376                                                           17    3301
XP_006713375                                                           17    3301
XP_005276078                                                           17    3301
NP_003481                                                              17    3301
NP_071904                                                              17    4312
XP_005266561                                                           17    4312
XP_005266560                                                           17    4312
XP_005266559                                                           17    4312
NP_001138537                                                           16    5631
NP_653294                                                              16    7355
NP_001273424                                                           16    7355
NP_062546                                                              16    7355
XP_005259813                                                           16    7355
XP_005259812                                                           16    7355
XP_006720675                                                           16    7355
XP_006720674                                                           16    7355
XP_006720672                                                           16    7355
XP_005254605                                                           16    7355
XP_005254604                                                           16    7355
NP_058649                                                              15    9605
NP_060136                                                              15    9605
NP_001254708                                                           15    9605
NP_001186548                                                           15    9605
NP_001116314                                                           15    9605
NP_861447                                                              15    9605
NP_001186550                                                           15    9605
NP_001186549                                                           15    9605
NP_001180481                                                           15    9605
NP_001803                                                              15    9605
NP_065996                                                              15    9605
XP_006724828                                                           15    9605
XP_006723248                                                           15    9605
XP_005258914                                                           15    9605
XP_005258915                                                           15    9605
XP_005270064                                                           15    9605
XP_005270062                                                           15    9605
XP_005270061                                                           15    9605
XP_005249999                                                           15    9605
XP_006715404                                                           15    9605
XP_006715403                                                           15    9605
XP_005266879                                                           15    9605
XP_006714128                                                           15    9605
XP_006714127                                                           15    9605
>NP_598328
          Length = 582

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LRKSFAS 7
           LRKSFAS
Sbjct: 572 LRKSFAS 578
>NP_001129246
          Length = 579

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LRKSFAS 7
           LRKSFAS
Sbjct: 569 LRKSFAS 575
>NP_008881
          Length = 705

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LRKSFAS 7
           LRKSFAS
Sbjct: 695 LRKSFAS 701
>XP_005276380
          Length = 232

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  RKSFASRS 9
          RKSF SRS
Sbjct: 27 RKSFLSRS 34
>XP_005251180
          Length = 229

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  RKSFASRS 9
          RKSF SRS
Sbjct: 27 RKSFLSRS 34
>XP_006713376
          Length = 325

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LRKSFAS 7
           LRKSFAS
Sbjct: 315 LRKSFAS 321
>XP_006713375
          Length = 421

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LRKSFAS 7
           LRKSFAS
Sbjct: 411 LRKSFAS 417
>XP_005276078
          Length = 232

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  RKSFASRS 9
          RKSF SRS
Sbjct: 27 RKSFLSRS 34
>NP_003481
          Length = 580

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LRKSFAS 7
           LRKSFAS
Sbjct: 570 LRKSFAS 576
>NP_071904
          Length = 1151

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LRKSFASRS 9
           L K FASRS
Sbjct: 729 LAKQFASRS 737
>XP_005266561
          Length = 861

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LRKSFASRS 9
           L K FASRS
Sbjct: 439 LAKQFASRS 447
>XP_005266560
          Length = 1037

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LRKSFASRS 9
           L K FASRS
Sbjct: 729 LAKQFASRS 737
>XP_005266559
          Length = 1077

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LRKSFASRS 9
           L K FASRS
Sbjct: 655 LAKQFASRS 663
>NP_001138537
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LRKSFASR 8
           L+K+FASR
Sbjct: 704 LQKAFASR 711
>NP_653294
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 187 KSFSSRS 193
>NP_001273424
          Length = 1083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 848 KSFSSRS 854
>NP_062546
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>XP_005259813
          Length = 331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 116 KSFSSRS 122
>XP_005259812
          Length = 402

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 187 KSFSSRS 193
>XP_006720675
          Length = 988

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 753 KSFSSRS 759
>XP_006720674
          Length = 988

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 753 KSFSSRS 759
>XP_006720672
          Length = 1053

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>XP_005254605
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>XP_005254604
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           KSF+SRS
Sbjct: 841 KSFSSRS 847
>NP_058649
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKSFA 6
           LRKSFA
Sbjct: 121 LRKSFA 126
>NP_060136
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RKSFASRS 9
           RKS ASRS
Sbjct: 553 RKSNASRS 560
>NP_001254708
          Length = 1058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 536 KSFTSRS 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 816 KSFTSRS 822
>NP_001186548
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 667 MRKSFATHT 675
>NP_001116314
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 656 MRKSFATHT 664
>NP_861447
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 667 MRKSFATHT 675
>NP_001186550
          Length = 827

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 552 MRKSFATHT 560
>NP_001186549
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 667 MRKSFATHT 675
>NP_001180481
          Length = 1090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 568 KSFTSRS 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 848 KSFTSRS 854
>NP_001803
          Length = 943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           +SFASRS
Sbjct: 310 QSFASRS 316
>NP_065996
          Length = 3184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RKSFASRS 9
            +KS ASRS
Sbjct: 2179 KKSLASRS 2186
>XP_006724828
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   RKSFASRS 9
           RKS ASRS
Sbjct: 504 RKSNASRS 511
>XP_006723248
          Length = 1060

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 538 KSFTSRS 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 818 KSFTSRS 824
>XP_005258914
          Length = 1092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 570 KSFTSRS 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 850 KSFTSRS 856
>XP_005258915
          Length = 1092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 570 KSFTSRS 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFASRS 9
           KSF SRS
Sbjct: 850 KSFTSRS 856
>XP_005270064
          Length = 1615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSFASRS 9
           +KS ASRS
Sbjct: 610 KKSLASRS 617
>XP_005270062
          Length = 3184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RKSFASRS 9
            +KS ASRS
Sbjct: 2179 KKSLASRS 2186
>XP_005270061
          Length = 3184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RKSFASRS 9
            +KS ASRS
Sbjct: 2179 KKSLASRS 2186
>XP_005249999
          Length = 576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LRKSFASR 8
           LRKSF+ R
Sbjct: 186 LRKSFSFR 193
>XP_006715404
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 563 MRKSFATHT 571
>XP_006715403
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 667 MRKSFATHT 675
>XP_005266879
          Length = 942

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKSFASRS 9
           +RKSFA+ +
Sbjct: 667 MRKSFATHT 675
>XP_006714128
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           +SFASRS
Sbjct: 97  QSFASRS 103
>XP_006714127
          Length = 935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KSFASRS 9
           +SFASRS
Sbjct: 310 QSFASRS 316
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.121    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 369,500
Number of extensions: 537
Number of successful extensions: 53
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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