Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           20     509
NP_001257437                                                           20     509
NP_006281                                                              20     509
XP_005267176                                                           20     509
NP_258428                                                              16    5631
NP_001269221                                                           16    5631
NP_009119                                                              16    5631
NP_001269220                                                           16    5631
NP_001269222                                                           16    5631
XP_005252339                                                           16    5631
NP_116198                                                              16    5631
NP_001268669                                                           16    7355
NP_005727                                                              16    7355
NP_079411                                                              16    7355
NP_001268668                                                           16    7355
XP_006722614                                                           16    7355
XP_005258412                                                           16    7355
XP_005258411                                                           16    7355
XP_005258409                                                           16    7355
XP_005258408                                                           16    7355
XP_006722613                                                           16    7355
XP_005258407                                                           16    7355
XP_005258406                                                           16    7355
XP_005258405                                                           16    7355
XP_005258404                                                           16    7355
XP_005258403                                                           16    7355
NP_079471                                                              15    9605
NP_004288                                                              15    9605
NP_835471                                                              15    9605
NP_001269914                                                           15    9605
NP_056985                                                              15    9605
NP_001269945                                                           15    9605
NP_001269944                                                           15    9605
NP_001269943                                                           15    9605
NP_001025036                                                           15    9605
XP_005269741                                                           15    9605
XP_006717813                                                           15    9605
XP_006717812                                                           15    9605
XP_006716560                                                           15    9605
XP_006716559                                                           15    9605
XP_006714210                                                           15    9605
XP_006714209                                                           15    9605
XP_006713692                                                           15    9605
XP_006713691                                                           15    9605
XP_006713690                                                           15    9605
XP_006713689                                                           15    9605
XP_006713688                                                           15    9605
XP_006713687                                                           15    9605
>NP_001257436
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           LRKALFST+
Sbjct: 122 LRKALFSTL 130
>NP_001257437
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           LRKALFST+
Sbjct: 122 LRKALFSTL 130
>NP_006281
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           LRKALFST+
Sbjct: 122 LRKALFSTL 130
>XP_005267176
          Length = 790

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           LRKALFST+
Sbjct: 122 LRKALFSTL 130
>NP_258428
          Length = 779

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKALFSTI 9
           RKALF T+
Sbjct: 423 RKALFGTM 430
>NP_001269221
          Length = 693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           +R+ALFS++
Sbjct: 210 MRRALFSSL 218
>NP_009119
          Length = 718

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           +R+ALFS++
Sbjct: 210 MRRALFSSL 218
>NP_001269220
          Length = 735

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           +R+ALFS++
Sbjct: 227 MRRALFSSL 235
>NP_001269222
          Length = 630

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           +R+ALFS++
Sbjct: 122 MRRALFSSL 130
>XP_005252339
          Length = 593

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LRKALFST 8
          LRK LF+T
Sbjct: 44 LRKVLFAT 51
>NP_116198
          Length = 593

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LRKALFST 8
          LRK LF+T
Sbjct: 44 LRKVLFAT 51
>NP_001268669
          Length = 1622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>NP_005727
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LRKALFSTI 9
           LR+ LFS I
Sbjct: 290 LRRTLFSNI 298
>NP_079411
          Length = 1439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>NP_001268668
          Length = 1422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_006722614
          Length = 1589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258412
          Length = 1401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258411
          Length = 1409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258409
          Length = 1430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258408
          Length = 1447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_006722613
          Length = 1523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258407
          Length = 1533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258406
          Length = 1559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258405
          Length = 1597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258404
          Length = 1605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>XP_005258403
          Length = 1614

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LRKALFS 7
           LR+ALFS
Sbjct: 123 LRRALFS 129
>NP_079471
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LRKALFST 8
           LRK +FST
Sbjct: 208 LRKFVFST 215
>NP_004288
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KALFSTI 9
           KALF TI
Sbjct: 248 KALFKTI 254
>NP_835471
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  KALFST 8
          KALFST
Sbjct: 81 KALFST 86
>NP_001269914
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LRKALFST 8
          LRK +FST
Sbjct: 42 LRKFVFST 49
>NP_056985
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKALF 6
           LRKALF
Sbjct: 138 LRKALF 143
>NP_001269945
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LRKALF 6
          LRKALF
Sbjct: 12 LRKALF 17
>NP_001269944
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKALF 6
           LRKALF
Sbjct: 138 LRKALF 143
>NP_001269943
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKALF 6
           LRKALF
Sbjct: 138 LRKALF 143
>NP_001025036
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRKALFSTI 9
           LR+A+FS +
Sbjct: 183 LRRAVFSDV 191
>XP_005269741
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKALF 6
           LRKALF
Sbjct: 138 LRKALF 143
>XP_006717813
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKALF 6
           LRKALF
Sbjct: 138 LRKALF 143
>XP_006717812
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LRKALF 6
           LRKALF
Sbjct: 138 LRKALF 143
>XP_006716560
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LRKALFST 8
          LRK +FST
Sbjct: 42 LRKFVFST 49
>XP_006716559
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LRKALFST 8
           LRK +FST
Sbjct: 98  LRKFVFST 105
>XP_006714210
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           +++A+FSTI
Sbjct: 874 MQEAVFSTI 882
>XP_006714209
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LRKALFSTI 9
           +++A+FSTI
Sbjct: 913 MQEAVFSTI 921
>XP_006713692
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KALFST 8
           KALFST
Sbjct: 118 KALFST 123
>XP_006713691
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KALFST 8
           KALFST
Sbjct: 118 KALFST 123
>XP_006713690
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KALFST 8
           KALFST
Sbjct: 212 KALFST 217
>XP_006713689
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KALFST 8
           KALFST
Sbjct: 212 KALFST 217
>XP_006713688
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KALFST 8
           KALFST
Sbjct: 212 KALFST 217
>XP_006713687
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KALFST 8
           KALFST
Sbjct: 212 KALFST 217
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.143    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 380,763
Number of extensions: 495
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package