Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_001005855                                                           17    4312
NP_006238                                                              16    7355
NP_733468                                                              16    7355
NP_653091                                                              16    7355
NP_001191213                                                           16    7355
NP_078963                                                              16    7355
NP_001078934                                                           16    7355
NP_001193816                                                           16    7355
NP_001322                                                              16    7355
NP_060857                                                              16    7355
NP_001164608                                                           16    7355
NP_001186131                                                           16    7355
NP_001186130                                                           16    7355
NP_001167531                                                           16    7355
NP_060254                                                              16    7355
XP_005252140                                                           16    7355
XP_005247836                                                           16    7355
XP_006713811                                                           16    7355
XP_006713810                                                           16    7355
XP_005247834                                                           16    7355
XP_005264902                                                           16    7355
XP_005264901                                                           16    7355
XP_005264900                                                           16    7355
NP_001439                                                              15    9605
NP_001058                                                              15    9605
NP_001189489                                                           15    9605
NP_059998                                                              15    9605
NP_997351                                                              15    9605
NP_001003698                                                           15    9605
NP_001161816                                                           15    9605
NP_001003699                                                           15    9605
NP_005094                                                              15    9605
XP_005257689                                                           15    9605
XP_005254268                                                           15    9605
XP_005271792                                                           15    9605
XP_005271791                                                           15    9605
XP_006715911                                                           15    9605
XP_006715910                                                           15    9605
XP_006715224                                                           15    9605
XP_005249329                                                           15    9605
XP_006715223                                                           15    9605
XP_006715222                                                           15    9605
XP_005249333                                                           15    9605
XP_006715221                                                           15    9605
XP_006715220                                                           15    9605
XP_005249332                                                           15    9605
XP_006715219                                                           15    9605
NP_689705                                                              15    9605
>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2921 LLANEKSDN 2929
>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2937 LLANEKSDN 2945
>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LRANEKSDN 9
            L ANEKSDN
Sbjct: 2938 LLANEKSDN 2946
>NP_001005855
          Length = 387

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           LR+NEKS +
Sbjct: 373 LRSNEKSSS 381
>NP_006238
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRANEKSDN 9
           LR N ++DN
Sbjct: 300 LRGNHETDN 308
>NP_733468
          Length = 2342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ANEKSDN 9
          ANE++DN
Sbjct: 8  ANEENDN 14
>NP_653091
          Length = 2316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  ANEKSDN 9
          ANE++DN
Sbjct: 8  ANEENDN 14
>NP_001191213
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LRANEKSDN 9
           LR N ++DN
Sbjct: 278 LRGNHETDN 286
>NP_078963
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 513 LESNQKSDD 521
>NP_001078934
          Length = 840

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           N+KSDN
Sbjct: 769 NQKSDN 774
>NP_001193816
          Length = 887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           N+KSDN
Sbjct: 816 NQKSDN 821
>NP_001322
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           N+KSDN
Sbjct: 870 NQKSDN 875
>NP_060857
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           + ++EKSDN
Sbjct: 464 MSSDEKSDN 472
>NP_001164608
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           + ++EKSDN
Sbjct: 450 MSSDEKSDN 458
>NP_001186131
          Length = 771

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 484 LESNQKSDD 492
>NP_001186130
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 513 LESNQKSDD 521
>NP_001167531
          Length = 700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LRANEKSDN 9
           L  +EKSDN
Sbjct: 310 LSEDEKSDN 318
>NP_060254
          Length = 764

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LRANEKSDN 9
           L  +EKSDN
Sbjct: 374 LSEDEKSDN 382
>XP_005252140
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           + ++EKSDN
Sbjct: 464 MSSDEKSDN 472
>XP_005247836
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 301 LESNQKSDD 309
>XP_006713811
          Length = 800

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 513 LESNQKSDD 521
>XP_006713810
          Length = 838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 512 LESNQKSDD 520
>XP_005247834
          Length = 839

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LRANEKSDN 9
           L +N+KSD+
Sbjct: 513 LESNQKSDD 521
>XP_005264902
          Length = 428

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LRANEKSDN 9
          L  +EKSDN
Sbjct: 38 LSEDEKSDN 46
>XP_005264901
          Length = 705

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LRANEKSDN 9
           L  +EKSDN
Sbjct: 310 LSEDEKSDN 318
>XP_005264900
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LRANEKSDN 9
           L  +EKSDN
Sbjct: 374 LSEDEKSDN 382
>NP_001439
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ANEKSDN 9
           ANEK+D+
Sbjct: 523 ANEKADS 529
>NP_001058
          Length = 1531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    NEKSDN 9
            NE+SDN
Sbjct: 1103 NEESDN 1108
>NP_001189489
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ANEKSDN 9
           AN+KS+N
Sbjct: 131 ANKKSEN 137
>NP_059998
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ANEKSDN 9
           AN+KS+N
Sbjct: 131 ANKKSEN 137
>NP_997351
          Length = 158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           N+KSDN
Sbjct: 112 NKKSDN 117
>NP_001003698
          Length = 1687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1484 RSSEKSDD 1491
>NP_001161816
          Length = 1687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1484 RSSEKSDD 1491
>NP_001003699
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>NP_005094
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           NEKS+N
Sbjct: 147 NEKSEN 152
>XP_005257689
          Length = 1519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    NEKSDN 9
            NE+SDN
Sbjct: 1091 NEESDN 1096
>XP_005254268
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  RANEKSDN 9
          RAN+KS++
Sbjct: 29 RANKKSES 36
>XP_005271792
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           NEKS+N
Sbjct: 147 NEKSEN 152
>XP_005271791
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   NEKSDN 9
           NEKS+N
Sbjct: 147 NEKSEN 152
>XP_006715911
          Length = 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ANEKSDN 9
           AN+KS+N
Sbjct: 100 ANKKSEN 106
>XP_006715910
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ANEKSDN 9
           AN+KS+N
Sbjct: 154 ANKKSEN 160
>XP_006715224
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_005249329
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_006715223
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_006715222
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_005249333
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_006715221
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_006715220
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_005249332
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>XP_006715219
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    RANEKSDN 9
            R++EKSD+
Sbjct: 1539 RSSEKSDD 1546
>NP_689705
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ANEKSDN 9
           ANE SDN
Sbjct: 603 ANEISDN 609
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.113    0.279 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 510,672
Number of extensions: 695
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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