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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1135
NP_005913                                                              18    1135
XP_005267046                                                           18    1135
XP_005267045                                                           18    1135
NP_001106849                                                           16    5631
NP_060663                                                              16    5631
XP_003960384                                                           15    9605
XP_003118572                                                           15    9605
XP_003119970                                                           15    9605

>NP_006715
          Length = 1558

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LQTLISQSK 9
            L+TLISQSK
Sbjct: 1227 LRTLISQSK 1235



>NP_005913
          Length = 1608

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LQTLISQSK 9
            L+TLISQSK
Sbjct: 1277 LRTLISQSK 1285



>XP_005267046
          Length = 1554

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LQTLISQSK 9
            L+TLISQSK
Sbjct: 1223 LRTLISQSK 1231



>XP_005267045
          Length = 1604

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LQTLISQSK 9
            L+TLISQSK
Sbjct: 1273 LRTLISQSK 1281



>NP_001106849
          Length = 1328

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LQTLISQSK 9
           +QTL+SQ K
Sbjct: 624 MQTLLSQLK 632



>NP_060663
          Length = 1268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LQTLISQSK 9
           +QTL+SQ K
Sbjct: 624 MQTLLSQLK 632



>XP_003960384
          Length = 884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LQTLISQ 7
           LQTLIS+
Sbjct: 432 LQTLISE 438



>XP_003118572
          Length = 884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LQTLISQ 7
           LQTLIS+
Sbjct: 432 LQTLISE 438



>XP_003119970
          Length = 884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LQTLISQ 7
           LQTLIS+
Sbjct: 432 LQTLISE 438



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.116    0.254 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 278,051
Number of extensions: 276
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)