Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_001268885                                                           17    3301
NP_443128                                                              17    3301
NP_055793                                                              17    4312
NP_054828                                                              17    4312
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_596870                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
NP_057195                                                              16    7355
NP_054829                                                              16    7355
XP_006721056                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_031373                                                              15    9605
NP_001264988                                                           15    9605
NP_001239056                                                           15    9605
NP_001005169                                                           15    9605
NP_001005170                                                           15    9605
XP_006720510                                                           15    9605
XP_005254321                                                           15    9605
XP_006714753                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LQIDSSISF 9
            LQIDSS+SF
Sbjct: 1653 LQIDSSVSF 1661
>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LQIDSSISF 9
            LQIDSS+SF
Sbjct: 1653 LQIDSSVSF 1661
>NP_001268885
          Length = 3631

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           LQ+ SSISF
Sbjct: 747 LQLGSSISF 755
>NP_443128
          Length = 3487

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           LQ+ SSISF
Sbjct: 707 LQLGSSISF 715
>NP_055793
          Length = 1222

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           + +DSSISF
Sbjct: 959 ITLDSSISF 967
>NP_054828
          Length = 1390

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           +Q+DSS+ F
Sbjct: 417 MQLDSSVRF 425
>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 936 QIESSIDF 943
>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 936 QIESSIDF 943
>NP_596870
          Length = 5604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 982 QIESSIDF 989
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 982 QIESSIDF 989
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 982 QIESSIDF 989
>NP_057195
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IDSSISF 9
           +DSS+SF
Sbjct: 207 LDSSVSF 213
>NP_054829
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           + IDS++SF
Sbjct: 109 IPIDSTVSF 117
>XP_006721056
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IDSSISF 9
           +DSS+SF
Sbjct: 207 LDSSVSF 213
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 983 QIESSIDF 990
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 983 QIESSIDF 990
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 982 QIESSIDF 989
>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   QIDSSISF 9
           QI+SSI F
Sbjct: 936 QIESSIDF 943
>NP_031373
          Length = 1137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           L +D+S+SF
Sbjct: 616 LVVDASLSF 624
>NP_001264988
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LQIDSSIS 8
           LQ+DSS S
Sbjct: 103 LQVDSSSS 110
>NP_001239056
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           L +D+S+SF
Sbjct: 541 LVVDASLSF 549
>NP_001005169
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  DSSISF 9
          DSSISF
Sbjct: 93 DSSISF 98
>NP_001005170
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DSSISF 9
           DSSISF
Sbjct: 119 DSSISF 124
>XP_006720510
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           L +D+S+SF
Sbjct: 541 LVVDASLSF 549
>XP_005254321
          Length = 1062

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LQIDSSISF 9
           L +D+S+SF
Sbjct: 541 LVVDASLSF 549
>XP_006714753
          Length = 221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LQIDSSIS 8
           LQ+DSS S
Sbjct: 103 LQVDSSSS 110
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.135    0.325 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 357,758
Number of extensions: 460
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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