Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_001399                                                              17    2528
XP_006710468                                                           17    2528
XP_005270637                                                           17    2528
NP_694950                                                              17    4312
NP_001121775                                                           17    4312
XP_006719830                                                           17    4312
XP_006719829                                                           17    4312
XP_005266318                                                           17    4312
XP_006717199                                                           17    4312
NP_003051                                                              16    7355
NP_036214                                                              16    7355
NP_002408                                                              16    7355
NP_001139438                                                           16    7355
XP_006717927                                                           16    7355
XP_006714739                                                           16    7355
XP_005272112                                                           16    7355
NP_001273983                                                           15    9605
NP_005156                                                              15    9605
NP_003430                                                              15    9605
XP_005258006                                                           15    9605
XP_005258004                                                           15    9605
XP_005250622                                                           15    9605
XP_006716175                                                           15    9605
>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LPRESAMAT 9
            LPRES+MAT
Sbjct: 1498 LPRESSMAT 1506
>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LPRESAMAT 9
            LPRES+MAT
Sbjct: 1293 LPRESSMAT 1301
>NP_001399
          Length = 2923

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LPRESAMAT 9
           +PR SA+AT
Sbjct: 266 MPRRSALAT 274
>XP_006710468
          Length = 2916

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LPRESAMAT 9
           +PR SA+AT
Sbjct: 266 MPRRSALAT 274
>XP_005270637
          Length = 2921

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LPRESAMAT 9
           +PR SA+AT
Sbjct: 266 MPRRSALAT 274
>NP_694950
          Length = 430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LPRESAMA 8
           LPRESA A
Sbjct: 345 LPRESAEA 352
>NP_001121775
          Length = 430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LPRESAMA 8
           LPRESA A
Sbjct: 345 LPRESAEA 352
>XP_006719830
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LPRESAMA 8
           LPRESA A
Sbjct: 345 LPRESAEA 352
>XP_006719829
          Length = 430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LPRESAMA 8
           LPRESA A
Sbjct: 345 LPRESAEA 352
>XP_005266318
          Length = 430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LPRESAMA 8
           LPRESA A
Sbjct: 345 LPRESAEA 352
>XP_006717199
          Length = 319

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  LPRESAMAT 9
          LPR  AMAT
Sbjct: 18 LPRVPAMAT 26
>NP_003051
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LPRESAMAT 9
           LPR  +MAT
Sbjct: 397 LPRRYSMAT 405
>NP_036214
          Length = 1151

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LPRESAMAT 9
           LP+++AMA+
Sbjct: 230 LPQQAAMAS 238
>NP_002408
          Length = 3256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    PRESAMA 8
            PRESA A
Sbjct: 2790 PRESAQA 2796
>NP_001139438
          Length = 2896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    PRESAMA 8
            PRESA A
Sbjct: 2430 PRESAQA 2436
>XP_006717927
          Length = 2406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    PRESAMA 8
            PRESA A
Sbjct: 1940 PRESAQA 1946
>XP_006714739
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LPRESAMAT 9
           LPR  +MAT
Sbjct: 193 LPRRYSMAT 201
>XP_005272112
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LPRESAMAT 9
           LPR  +MAT
Sbjct: 421 LPRRYSMAT 429
>NP_001273983
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PRESAMAT 9
           PR+ AMA+
Sbjct: 173 PRDQAMAS 180
>NP_005156
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PRESAMAT 9
           P E AMAT
Sbjct: 246 PEEIAMAT 253
>NP_003430
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PRESAMAT 9
           PR+ AMA+
Sbjct: 209 PRDQAMAS 216
>XP_005258006
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PRESAMAT 9
           P E AMAT
Sbjct: 246 PEEIAMAT 253
>XP_005258004
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PRESAMAT 9
           P E AMAT
Sbjct: 333 PEEIAMAT 340
>XP_005250622
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PRESAMAT 9
           PR+ AMA+
Sbjct: 209 PRDQAMAS 216
>XP_006716175
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   PRESAMAT 9
           PR+ AMA+
Sbjct: 209 PRDQAMAS 216
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.114    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 455,647
Number of extensions: 329
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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