Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              19     665
NP_573439                                                              17    4312
NP_573438                                                              17    4312
NP_001181930                                                           17    4312
NP_005695                                                              17    4312
NP_001258                                                              17    4312
XP_006710336                                                           17    4312
XP_006710335                                                           17    4312
XP_006710334                                                           17    4312
XP_006710333                                                           17    4312
XP_006710332                                                           17    4312
NP_001032                                                              16    5631
NP_001098714                                                           16    5631
XP_006722401                                                           16    5631
XP_006722400                                                           16    5631
XP_006722399                                                           16    5631
XP_006722398                                                           16    5631
NP_002836                                                              16    5631
NP_004692                                                              16    7355
NP_055571                                                              15    9605
NP_001008938                                                           15    9605
NP_694992                                                              15    9605
NP_689725                                                              15    9605
NP_115597                                                              15    9605
XP_006724215                                                           15    9605
XP_006724214                                                           15    9605
XP_006724213                                                           15    9605
XP_006724212                                                           15    9605
XP_006723946                                                           15    9605
XP_005260632                                                           15    9605
XP_005260631                                                           15    9605
XP_005260630                                                           15    9605

>NP_003290
          Length = 803

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  LNSSQIREL 9
          LN+SQIREL
Sbjct: 61 LNASQIREL 69



>NP_573439
          Length = 1436

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1162 NSSQLRE 1168



>NP_573438
          Length = 1440

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1168 NSSQLRE 1174



>NP_001181930
          Length = 1433

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1159 NSSQLRE 1165



>NP_005695
          Length = 1446

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1172 NSSQLRE 1178



>NP_001258
          Length = 359

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LNSSQIREL 9
           LN+++IREL
Sbjct: 154 LNNNKIREL 162



>XP_006710336
          Length = 1177

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   NSSQIRE 8
           NSSQ+RE
Sbjct: 903 NSSQLRE 909



>XP_006710335
          Length = 1370

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1096 NSSQLRE 1102



>XP_006710334
          Length = 1292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1018 NSSQLRE 1024



>XP_006710333
          Length = 1439

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1165 NSSQLRE 1171



>XP_006710332
          Length = 1442

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQ+RE
Sbjct: 1168 NSSQLRE 1174



>NP_001032
          Length = 1827

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NSSQIREL 9
            N+S++REL
Sbjct: 1235 NTSEVREL 1242



>NP_001098714
          Length = 1465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQI+E
Sbjct: 1195 NSSQIKE 1201



>XP_006722401
          Length = 1432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQI+E
Sbjct: 1162 NSSQIKE 1168



>XP_006722400
          Length = 1469

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQI+E
Sbjct: 1199 NSSQIKE 1205



>XP_006722399
          Length = 1477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQI+E
Sbjct: 1207 NSSQIKE 1213



>XP_006722398
          Length = 1494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQI+E
Sbjct: 1224 NSSQIKE 1230



>NP_002836
          Length = 1452

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    NSSQIRE 8
            NSSQI+E
Sbjct: 1182 NSSQIKE 1188



>NP_004692
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SSQIREL 9
           SSQIRE+
Sbjct: 243 SSQIREM 249



>NP_055571
          Length = 1972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNSSQIREL 9
           +NS Q++EL
Sbjct: 206 INSVQLKEL 214



>NP_001008938
          Length = 2032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNSSQIREL 9
           +NS Q++EL
Sbjct: 206 INSVQLKEL 214



>NP_694992
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNSSQIREL 9
           LN++ +REL
Sbjct: 92  LNNNHLREL 100



>NP_689725
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LNSSQIRE 8
           LNSSQI +
Sbjct: 585 LNSSQISQ 592



>NP_115597
          Length = 2715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NSSQIRE 8
           NS+Q+RE
Sbjct: 457 NSNQLRE 463



>XP_006724215
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LNSSQIRE 8
           LNSSQI +
Sbjct: 454 LNSSQISQ 461



>XP_006724214
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LNSSQIRE 8
           LNSSQI +
Sbjct: 472 LNSSQISQ 479



>XP_006724213
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LNSSQIRE 8
           LNSSQI +
Sbjct: 532 LNSSQISQ 539



>XP_006724212
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LNSSQIRE 8
           LNSSQI +
Sbjct: 585 LNSSQISQ 592



>XP_006723946
          Length = 1414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NSSQIRE 8
           NS+Q+RE
Sbjct: 457 NSNQLRE 463



>XP_005260632
          Length = 2377

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NSSQIRE 8
           NS+Q+RE
Sbjct: 457 NSNQLRE 463



>XP_005260631
          Length = 2667

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NSSQIRE 8
           NS+Q+RE
Sbjct: 457 NSNQLRE 463



>XP_005260630
          Length = 2693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NSSQIRE 8
           NS+Q+RE
Sbjct: 435 NSNQLRE 441



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.124    0.284 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 386,976
Number of extensions: 544
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)