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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     509
NP_055481                                                              18    1935
NP_001182485                                                           18    1935
NP_001182484                                                           18    1935
NP_001008781                                                           16    5631
NP_001095939                                                           16    7355
XP_006720705                                                           16    7355
XP_005270106                                                           16    7355
NP_001094874                                                           16    7355
NP_001367                                                              15    9605
NP_001420                                                              15    9605
NP_001600                                                              15    9605
NP_001274736                                                           15    9605
XP_006724228                                                           15    9605

>NP_115803
          Length = 1156

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LNQSRMGIN 9
            LNQSR GIN
Sbjct: 1069 LNQSRTGIN 1077




 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    LNQSRMGIN 9
            LNQS  G+N
Sbjct: 1062 LNQSGTGLN 1070



>NP_055481
          Length = 625

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNQSRMGIN 9
           +NQS MG+N
Sbjct: 572 MNQSMMGMN 580



>NP_001182485
          Length = 625

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNQSRMGIN 9
           +NQS MG+N
Sbjct: 572 MNQSMMGMN 580



>NP_001182484
          Length = 643

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNQSRMGIN 9
           +NQS MG+N
Sbjct: 590 MNQSMMGMN 598



>NP_001008781
          Length = 4557

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  NQSRMGI 8
          +QSRMGI
Sbjct: 64 SQSRMGI 70



>NP_001095939
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNQSRMGI 8
           +NQSRM +
Sbjct: 271 MNQSRMDV 278



>XP_006720705
          Length = 508

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   LNQSRMGI 8
           +NQ+R+G+
Sbjct: 306 MNQARLGL 313



>XP_005270106
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNQSRMGI 8
           +NQSRM +
Sbjct: 271 MNQSRMDV 278



>NP_001094874
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   LNQSRMGI 8
           +NQ+R+G+
Sbjct: 249 MNQARLGL 256



>NP_001367
          Length = 4646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LNQSRMGIN 9
           LNQ + G+N
Sbjct: 242 LNQLQSGVN 250



>NP_001420
          Length = 2414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LNQSRMGIN 9
           +NQ  MG+N
Sbjct: 158 VNQPAMGMN 166



>NP_001600
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNQSRMGI 8
           LN+ R+GI
Sbjct: 290 LNEGRIGI 297



>NP_001274736
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LNQSRMGIN 9
           LNQ  +G+N
Sbjct: 135 LNQRDLGLN 143



>XP_006724228
          Length = 2388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LNQSRMGIN 9
           +NQ  MG+N
Sbjct: 158 VNQPAMGMN 166



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.131    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,767
Number of extensions: 233
Number of successful extensions: 15
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)