Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_443732                                                              17    4312
NP_002262                                                              16    5631
NP_037385                                                              16    5631
NP_001018067                                                           16    5631
NP_056965                                                              16    5631
XP_005254110                                                           16    5631
XP_005254109                                                           16    5631
XP_005254106                                                           16    5631
XP_006718243                                                           16    5631
XP_006718242                                                           16    5631
XP_006718241                                                           16    5631
XP_006718240                                                           16    5631
NP_001005619                                                           15    9605
NP_001005731                                                           15    9605
NP_000204                                                              15    9605
NP_006695                                                              15    9605
NP_001124384                                                           15    9605
XP_005257369                                                           15    9605
XP_005257368                                                           15    9605
XP_006721933                                                           15    9605
XP_006721932                                                           15    9605
XP_006721931                                                           15    9605
XP_006721930                                                           15    9605
XP_005257366                                                           15    9605
XP_006721929                                                           15    9605

>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LNKMFRQLA 9
           LNKMF QLA
Sbjct: 130 LNKMFCQLA 138



>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LNKMFRQLA 9
          LNKMF QLA
Sbjct: 91 LNKMFCQLA 99



>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LNKMFRQLA 9
           LNKMF QLA
Sbjct: 130 LNKMFCQLA 138



>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LNKMFRQLA 9
           LNKMF QLA
Sbjct: 130 LNKMFCQLA 138



>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LNKMFRQLA 9
          LNKMF QLA
Sbjct: 91 LNKMFCQLA 99



>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LNKMFRQLA 9
          LNKMF QLA
Sbjct: 91 LNKMFCQLA 99



>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LNKMFRQLA 9
          LNKMF QLA
Sbjct: 91 LNKMFCQLA 99



>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LNKMFRQLA 9
           LNKMF QLA
Sbjct: 130 LNKMFCQLA 138



>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LNKMFRQLA 9
          LNKMF QLA
Sbjct: 91 LNKMFCQLA 99



>NP_443732
          Length = 358

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KMFRQLA 9
           KMFRQL+
Sbjct: 115 KMFRQLS 121



>NP_002262
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LNKMFRQLA 9
           LN M RQLA
Sbjct: 289 LNNMQRQLA 297



>NP_037385
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LNKMFRQL 8
          LN+MFR++
Sbjct: 47 LNEMFREV 54



>NP_001018067
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LNKMFRQL 8
          LN+MFR++
Sbjct: 47 LNEMFREV 54



>NP_056965
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LNKMFRQL 8
          LN+MFR++
Sbjct: 47 LNEMFREV 54



>XP_005254110
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LNKMFRQLA 9
           LN M RQLA
Sbjct: 289 LNNMQRQLA 297



>XP_005254109
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LNKMFRQLA 9
           LN M RQLA
Sbjct: 289 LNNMQRQLA 297



>XP_005254106
          Length = 1115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LNKMFRQLA 9
           LN M RQLA
Sbjct: 289 LNNMQRQLA 297



>XP_006718243
          Length = 216

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LNKMFRQL 8
           LN+MFR++
Sbjct: 110 LNEMFREV 117



>XP_006718242
          Length = 258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LNKMFRQL 8
           LN+MFR++
Sbjct: 110 LNEMFREV 117



>XP_006718241
          Length = 350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LNKMFRQL 8
          LN+MFR++
Sbjct: 47 LNEMFREV 54



>XP_006718240
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LNKMFRQL 8
           LN+MFR++
Sbjct: 110 LNEMFREV 117



>NP_001005619
          Length = 1805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 851 LNEVYRQIS 859



>NP_001005731
          Length = 1752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 851 LNEVYRQIS 859



>NP_000204
          Length = 1822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 851 LNEVYRQIS 859



>NP_006695
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   LNKMFRQL 8
           LN++F+Q+
Sbjct: 277 LNRLFQQI 284



>NP_001124384
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   LNKMFRQL 8
           LN++F+Q+
Sbjct: 309 LNRLFQQI 316



>XP_005257369
          Length = 1752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 851 LNEVYRQIS 859



>XP_005257368
          Length = 1875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 851 LNEVYRQIS 859



>XP_006721933
          Length = 1787

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 886 LNEVYRQIS 894



>XP_006721932
          Length = 1821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 797 LNEVYRQIS 805



>XP_006721931
          Length = 1840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 886 LNEVYRQIS 894



>XP_006721930
          Length = 1857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 886 LNEVYRQIS 894



>XP_005257366
          Length = 1875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 851 LNEVYRQIS 859



>XP_006721929
          Length = 1910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   LNKMFRQLA 9
           LN+++RQ++
Sbjct: 886 LNEVYRQIS 894



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.141    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,530
Number of extensions: 596
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)