Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275685                                                           17    3301
NP_001028721                                                           17    3301
NP_054892                                                              17    3301
NP_112217                                                              17    3301
NP_477512                                                              17    4312
XP_005260209                                                           17    4312
NP_005227                                                              16    7355
NP_001271230                                                           15    9605
NP_149985                                                              15    9605
NP_001271231                                                           15    9605
NP_061155                                                              15    9605
NP_848627                                                              15    9605
NP_001107033                                                           15    9605
XP_006715810                                                           15    9605
XP_006715809                                                           15    9605
XP_006714654                                                           15    9605
XP_006714653                                                           15    9605
XP_006714652                                                           15    9605
>NP_001275685
          Length = 329

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LNISQKM 7
           LN+SQKM
Sbjct: 121 LNVSQKM 127
>NP_001028721
          Length = 329

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LNISQKM 7
           LN+SQKM
Sbjct: 121 LNVSQKM 127
>NP_054892
          Length = 329

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LNISQKM 7
           LN+SQKM
Sbjct: 121 LNVSQKM 127
>NP_112217
          Length = 1594

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNISQKMEI 9
           +NISQK E+
Sbjct: 214 VNISQKQEL 222
>NP_477512
          Length = 454

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  NISQKMEI 9
          N+SQ ME+
Sbjct: 74 NVSQSMEV 81
>XP_005260209
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NISQKMEI 9
           N+SQ ME+
Sbjct: 98  NVSQSMEV 105
>NP_005227
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISQKMEI 9
           IS+KMEI
Sbjct: 357 ISEKMEI 363
>NP_001271230
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNISQKME 8
           +NI QKM+
Sbjct: 677 VNIKQKMQ 684
>NP_149985
          Length = 549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  LNISQKMEI 9
          LN+ +KM++
Sbjct: 10 LNLEEKMKV 18
>NP_001271231
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNISQKME 8
           +NI QKM+
Sbjct: 676 VNIKQKMQ 683
>NP_061155
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNISQKME 8
           +NI QKM+
Sbjct: 714 VNIKQKMQ 721
>NP_848627
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNISQKMEI 9
           LNIS+K+ +
Sbjct: 233 LNISEKLTL 241
>NP_001107033
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNISQKMEI 9
           LNIS+K+ +
Sbjct: 233 LNISEKLTL 241
>XP_006715810
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNISQKME 8
           +NI QKM+
Sbjct: 677 VNIKQKMQ 684
>XP_006715809
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LNISQKME 8
           +NI QKM+
Sbjct: 714 VNIKQKMQ 721
>XP_006714654
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNISQKMEI 9
           LNIS+K+ +
Sbjct: 233 LNISEKLTL 241
>XP_006714653
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNISQKMEI 9
           LNIS+K+ +
Sbjct: 233 LNISEKLTL 241
>XP_006714652
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LNISQKMEI 9
           LNIS+K+ +
Sbjct: 233 LNISEKLTL 241
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.129    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 370,616
Number of extensions: 464
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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