Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_001274103                                                           15    9605
NP_000343                                                              15    9605
NP_001243183                                                           15    9605
NP_001243181                                                           15    9605
NP_001965                                                              15    9605
NP_001243182                                                           15    9605
NP_001243184                                                           15    9605
XP_006718349                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1631 NAQRSTLK 1638
>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1647 NAQRSTLK 1654
>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    NAQRSTLK 9
            NAQRSTLK
Sbjct: 1648 NAQRSTLK 1655
>NP_001274103
          Length = 1582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AQRSTLK 9
           AQRSTL+
Sbjct: 484 AQRSTLE 490
>NP_000343
          Length = 1581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AQRSTLK 9
           AQRSTL+
Sbjct: 484 AQRSTLE 490
>NP_001243183
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNAQRSTLK 9
           +NA+ STLK
Sbjct: 642 VNAEVSTLK 650
>NP_001243181
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNAQRSTLK 9
           +NA+ STLK
Sbjct: 764 VNAEVSTLK 772
>NP_001965
          Length = 886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNAQRSTLK 9
           +NA+ STLK
Sbjct: 783 VNAEVSTLK 791
>NP_001243182
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNAQRSTLK 9
           +NA+ STLK
Sbjct: 718 VNAEVSTLK 726
>NP_001243184
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LNAQRSTLK 9
           +NA+ STLK
Sbjct: 606 VNAEVSTLK 614
>XP_006718349
          Length = 1646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AQRSTLK 9
           AQRSTL+
Sbjct: 483 AQRSTLE 489
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.116    0.274 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 298,037
Number of extensions: 433
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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