Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_001010904                                                           17    4312
NP_079229                                                              16    5631
XP_005253995                                                           16    5631
XP_006719669                                                           16    5631
NP_060897                                                              16    7355
NP_057302                                                              16    7355
XP_006725262                                                           16    7355
XP_006725261                                                           16    7355
XP_006725260                                                           16    7355
XP_005255434                                                           16    7355
XP_005255433                                                           16    7355
XP_005255432                                                           16    7355
XP_005248593                                                           16    7355
XP_006714714                                                           16    7355
XP_006714713                                                           16    7355
XP_005248591                                                           16    7355
NP_005049                                                              15    9605
NP_001269                                                              15    9605
NP_872302                                                              15    9605
NP_001006121                                                           15    9605
NP_689798                                                              15    9605
NP_002080                                                              15    9605
NP_001006118                                                           15    9605
XP_005262647                                                           15    9605
XP_005262646                                                           15    9605
XP_005262645                                                           15    9605
XP_005262644                                                           15    9605
XP_005262643                                                           15    9605
XP_005262642                                                           15    9605
XP_005262641                                                           15    9605
XP_005262640                                                           15    9605
XP_005262634                                                           15    9605
XP_005262633                                                           15    9605
XP_005262632                                                           15    9605
XP_005262631                                                           15    9605
XP_005262574                                                           15    9605
XP_005262573                                                           15    9605
XP_005262572                                                           15    9605
XP_005262571                                                           15    9605
XP_005262570                                                           15    9605
XP_005262569                                                           15    9605
XP_005262568                                                           15    9605
XP_006719292                                                           15    9605
NP_777592                                                              15    9605
NP_001006117                                                           15    9605
NP_001006120                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LMVEKMLKA 9
            L+VEKMLKA
Sbjct: 3445 LVVEKMLKA 3453
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LMVEKMLKA 9
            L+VEKMLKA
Sbjct: 3445 LVVEKMLKA 3453
>NP_001010904
          Length = 288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  LMVEKMLKA 9
          L++EKMLK+
Sbjct: 11 LILEKMLKS 19
>NP_079229
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MVEKMLK 8
           MVEKM+K
Sbjct: 180 MVEKMVK 186
>XP_005253995
          Length = 836

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  MVEKMLK 8
          MVEKM+K
Sbjct: 44 MVEKMVK 50
>XP_006719669
          Length = 944

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MVEKMLK 8
           MVEKM+K
Sbjct: 152 MVEKMVK 158
>NP_060897
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LMVEKMLK 8
           L++EK+LK
Sbjct: 246 LIIEKLLK 253
>NP_057302
          Length = 870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MVEKMLKA 9
           + EKMLKA
Sbjct: 360 VTEKMLKA 367
>XP_006725262
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LMVEKMLK 8
          L++EK+LK
Sbjct: 34 LIIEKLLK 41
>XP_006725261
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LMVEKMLK 8
          L++EK+LK
Sbjct: 34 LIIEKLLK 41
>XP_006725260
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LMVEKMLK 8
           L++EK+LK
Sbjct: 216 LIIEKLLK 223
>XP_005255434
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LMVEKMLK 8
          L++EK+LK
Sbjct: 34 LIIEKLLK 41
>XP_005255433
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  LMVEKMLK 8
          L++EK+LK
Sbjct: 34 LIIEKLLK 41
>XP_005255432
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LMVEKMLK 8
           L++EK+LK
Sbjct: 216 LIIEKLLK 223
>XP_005248593
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MVEKMLKA 9
           + EKMLKA
Sbjct: 374 VTEKMLKA 381
>XP_006714714
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MVEKMLKA 9
           + EKMLKA
Sbjct: 284 VTEKMLKA 291
>XP_006714713
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MVEKMLKA 9
           + EKMLKA
Sbjct: 374 VTEKMLKA 381
>XP_005248591
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MVEKMLKA 9
           + EKMLKA
Sbjct: 374 VTEKMLKA 381
>NP_005049
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>NP_001269
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EKMLKA 9
           EKMLKA
Sbjct: 505 EKMLKA 510
>NP_872302
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EKMLKA 9
           EKMLKA
Sbjct: 392 EKMLKA 397
>NP_001006121
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>NP_689798
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>NP_002080
          Length = 107

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MVEKMLK 8
           ++EKMLK
Sbjct: 96  IIEKMLK 102
>NP_001006118
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>XP_005262647
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262646
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262645
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262644
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262643
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262642
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>XP_005262641
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262640
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262634
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>XP_005262633
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262632
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262631
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262574
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262573
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>XP_005262572
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262571
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262570
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>XP_005262569
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_005262568
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 28 EKMLKA 33
>XP_006719292
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EKMLKA 9
           EKMLKA
Sbjct: 228 EKMLKA 233
>NP_777592
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MVEKMLK 8
          M+E+MLK
Sbjct: 49 MIEQMLK 55
>NP_001006117
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
>NP_001006120
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EKMLKA 9
          EKMLKA
Sbjct: 21 EKMLKA 26
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.132    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 347,808
Number of extensions: 506
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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