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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              18    1935
NP_004566                                                              16    5631
NP_001138537                                                           15    9605
NP_997374                                                              15    9605
XP_006725521                                                           15    9605
XP_005276858                                                           15    9605
XP_006719760                                                           15    9605
XP_006719759                                                           15    9605
XP_006719758                                                           15    9605
XP_005254048                                                           15    9605
XP_006719757                                                           15    9605
XP_006714693                                                           15    9605
NP_997194                                                              15    9605

>NP_000312
          Length = 928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LMMSLNSAS 9
           LMM LNSAS
Sbjct: 385 LMMILNSAS 393



>NP_004566
          Length = 409

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 LMMSLNS 7
         +MMSLNS
Sbjct: 2 MMMSLNS 8



>NP_001138537
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MSLNSAS 9
           MSLNSA+
Sbjct: 296 MSLNSAA 302



>NP_997374
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   MSLNSAS 9
           MSLNSA+
Sbjct: 296 MSLNSAA 302



>XP_006725521
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MMSLNSAS 9
           MM+L+SAS
Sbjct: 782 MMTLHSAS 789



>XP_005276858
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MMSLNSAS 9
           MM+L+SAS
Sbjct: 782 MMTLHSAS 789



>XP_006719760
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MMSLNS 7
           MMSLNS
Sbjct: 426 MMSLNS 431



>XP_006719759
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MMSLNS 7
           MMSLNS
Sbjct: 499 MMSLNS 504



>XP_006719758
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MMSLNS 7
           MMSLNS
Sbjct: 547 MMSLNS 552



>XP_005254048
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MMSLNS 7
           MMSLNS
Sbjct: 543 MMSLNS 548



>XP_006719757
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MMSLNS 7
           MMSLNS
Sbjct: 547 MMSLNS 552



>XP_006714693
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MMSLNSAS 9
           MM+L+SAS
Sbjct: 782 MMTLHSAS 789



>NP_997194
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MMSLNS 7
           MMSLNS
Sbjct: 495 MMSLNS 500



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.108    0.236 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 232,952
Number of extensions: 174
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)