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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005381                                                              16    5631
NP_065815                                                              16    7355
XP_005255515                                                           16    7355
NP_066357                                                              15    9605
NP_000992                                                              15    9605
NP_001186902                                                           15    9605
NP_055787                                                              15    9605

>NP_005381
          Length = 388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LMLAIRRMK 9
          +ML +RRM+
Sbjct: 65 MMLTVRRME 73



>NP_065815
          Length = 1431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LMLAIRRM 8
           LMLA+R++
Sbjct: 595 LMLAVRKL 602



>XP_005255515
          Length = 1360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LMLAIRRM 8
           LMLA+R++
Sbjct: 524 LMLAVRKL 531



>NP_066357
          Length = 142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MLAIRRMK 9
           MLAI+R K
Sbjct: 118 MLAIKRCK 125



>NP_000992
          Length = 106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  MLAIRRMK 9
          MLAI+R K
Sbjct: 82 MLAIKRCK 89



>NP_001186902
          Length = 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MLAIRRMK 9
           MLAI+R K
Sbjct: 118 MLAIKRCK 125



>NP_055787
          Length = 676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LMLAIRRM 8
           LMLA+R M
Sbjct: 645 LMLAVRDM 652



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.357    0.153    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 257,069
Number of extensions: 145
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.1 bits)
S2: 28 (15.4 bits)