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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           21     229
NP_004561                                                              21     229
NP_001275703                                                           21     229
XP_005253450                                                           21     229
XP_006719151                                                           21     229
NP_005872                                                              17    4312
NP_001258855                                                           17    4312
NP_001116429                                                           17    4312
NP_078959                                                              17    4312
XP_006722147                                                           17    4312

>NP_001275701
          Length = 1486

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LMEMMLYAG 9
            L+EMMLYAG
Sbjct: 1157 LLEMMLYAG 1165



>NP_004561
          Length = 1445

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LMEMMLYAG 9
            L+EMMLYAG
Sbjct: 1116 LLEMMLYAG 1124



>NP_001275703
          Length = 1264

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LMEMMLYAG 9
           L+EMMLYAG
Sbjct: 935 LLEMMLYAG 943



>XP_005253450
          Length = 1486

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LMEMMLYAG 9
            L+EMMLYAG
Sbjct: 1157 LLEMMLYAG 1165



>XP_006719151
          Length = 1486

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LMEMMLYAG 9
            L+EMMLYAG
Sbjct: 1157 LLEMMLYAG 1165



>NP_005872
          Length = 412

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MMLYAG 9
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001258855
          Length = 335

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MMLYAG 9
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_001116429
          Length = 365

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  MMLYAG 9
          MMLYAG
Sbjct: 74 MMLYAG 79



>NP_078959
          Length = 360

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LMEMMLYA 8
           +ME MLYA
Sbjct: 252 IMEAMLYA 259



>XP_006722147
          Length = 245

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LMEMMLYA 8
           +ME MLYA
Sbjct: 137 IMEAMLYA 144



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.142    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 364,854
Number of extensions: 394
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)