Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              18    1135
NP_001091680                                                           18    1135
NP_001091679                                                           18    1135
XP_006713048                                                           18    1135
XP_005264943                                                           18    1135
NP_859070                                                              17    2528
XP_005245884                                                           17    2528
XP_005245882                                                           17    2528
XP_006710614                                                           17    2528
XP_005245881                                                           17    2528
XP_005245880                                                           17    2528
XP_006710613                                                           17    2528
XP_005245879                                                           17    2528
XP_006713047                                                           17    3301
XP_006713046                                                           17    3301
NP_001035232                                                           16    5631
NP_066287                                                              16    5631
NP_001035233                                                           16    5631
XP_005246813                                                           16    5631
XP_005246812                                                           16    5631
XP_005246811                                                           16    5631
XP_005246810                                                           16    5631
XP_005246808                                                           16    5631
XP_005246807                                                           16    5631
NP_113656                                                              16    7355
NP_001017440                                                           16    7355
XP_006721061                                                           16    7355
XP_006716209                                                           16    7355
XP_006710064                                                           16    7355
NP_003931                                                              15    9605
NP_005384                                                              15    9605
NP_001156729                                                           15    9605
NP_001001794                                                           15    9605
XP_005261972                                                           15    9605
XP_005261971                                                           15    9605
>NP_001895
          Length = 781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LMELGMAME 9
          LMEL MAME
Sbjct: 7  LMELDMAME 15
>NP_001091680
          Length = 781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LMELGMAME 9
          LMEL MAME
Sbjct: 7  LMELDMAME 15
>NP_001091679
          Length = 781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LMELGMAME 9
          LMEL MAME
Sbjct: 7  LMELDMAME 15
>XP_006713048
          Length = 720

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LMELGMAME 9
          LMEL MAME
Sbjct: 7  LMELDMAME 15
>XP_005264943
          Length = 781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  LMELGMAME 9
          LMEL MAME
Sbjct: 7  LMELDMAME 15
>NP_859070
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_005245884
          Length = 594

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 278 LMELGNALE 286
>XP_005245882
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_006710614
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_005245881
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_005245880
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_006710613
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_005245879
          Length = 634

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LMELG A+E
Sbjct: 318 LMELGNALE 326
>XP_006713047
          Length = 774

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MELGMAME 9
         MEL MAME
Sbjct: 1 MELDMAME 8
>XP_006713046
          Length = 774

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2 MELGMAME 9
         MEL MAME
Sbjct: 1 MELDMAME 8
>NP_001035232
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 835 LMELGLA 841
>NP_066287
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 835 LMELGLA 841
>NP_001035233
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 835 LMELGLA 841
>XP_005246813
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 101 LMELGLA 107
>XP_005246812
          Length = 1754

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 584 LMELGLA 590
>XP_005246811
          Length = 1995

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 825 LMELGLA 831
>XP_005246810
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 835 LMELGLA 841
>XP_005246808
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 835 LMELGLA 841
>XP_005246807
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LMELGMA 7
           LMELG+A
Sbjct: 835 LMELGLA 841
>NP_113656
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   ELGMAM 8
           ELGMAM
Sbjct: 102 ELGMAM 107
>NP_001017440
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ELGMAM 8
          ELGMAM
Sbjct: 60 ELGMAM 65
>XP_006721061
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           L+ELG+ +E
Sbjct: 357 LLELGLRLE 365
>XP_006716209
          Length = 134

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  ELGMAM 8
          ELGMAM
Sbjct: 60 ELGMAM 65
>XP_006710064
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           L+ELG+ +E
Sbjct: 357 LLELGLRLE 365
>NP_003931
          Length = 863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           LM+L +AME
Sbjct: 498 LMQLPVAME 506
>NP_005384
          Length = 1909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           ++ELG +ME
Sbjct: 319 MVELGASME 327
>NP_001156729
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LMELGMAME 9
           ++ELG +ME
Sbjct: 342 MVELGASME 350
>NP_001001794
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MELGMAME 9
          +ELG A+E
Sbjct: 52 LELGQALE 59
>XP_005261972
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MELGMAME 9
          +ELG A+E
Sbjct: 52 LELGQALE 59
>XP_005261971
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MELGMAME 9
          +ELG A+E
Sbjct: 52 LELGQALE 59
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.134    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 381,442
Number of extensions: 450
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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