Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              17    2528
NP_444254                                                              17    2528
NP_444253                                                              17    2528
NP_444255                                                              17    2528
NP_001248359                                                           17    2528
NP_001136126                                                           17    2528
NP_001136125                                                           17    2528
NP_001129514                                                           17    2528
NP_079543                                                              17    2528
XP_005247551                                                           17    2528
XP_005247550                                                           17    2528
XP_006713712                                                           17    2528
XP_005247549                                                           17    2528
NP_114119                                                              17    4312
XP_006713840                                                           17    4312
XP_006713839                                                           17    4312
NP_758424                                                              16    7355
NP_061142                                                              16    7355
NP_689914                                                              16    7355
NP_066990                                                              15    9605
NP_001103444                                                           15    9605
NP_001159681                                                           15    9605
NP_001159680                                                           15    9605
NP_009217                                                              15    9605
XP_005268428                                                           15    9605
XP_005268427                                                           15    9605
XP_005268426                                                           15    9605
XP_006714818                                                           15    9605
>NP_444256
          Length = 1794

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 831 LLGKKVSTK 839
>NP_444254
          Length = 1845

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 831 LLGKKVSTK 839
>NP_444253
          Length = 1914

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 900 LLGKKVSTK 908
>NP_444255
          Length = 1863

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 900 LLGKKVSTK 908
>NP_001248359
          Length = 199

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LVKKVSTK 9
           L+KKVSTK
Sbjct: 170 LLKKVSTK 177
>NP_001136126
          Length = 161

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LVKKVSTK 9
           L+KKVSTK
Sbjct: 132 LLKKVSTK 139
>NP_001136125
          Length = 166

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LVKKVSTK 9
           L+KKVSTK
Sbjct: 137 LLKKVSTK 144
>NP_001129514
          Length = 586

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LVKKVSTK 9
           L+KKVSTK
Sbjct: 170 LLKKVSTK 177
>NP_079543
          Length = 525

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LVKKVSTK 9
           L+KKVSTK
Sbjct: 109 LLKKVSTK 116
>XP_005247551
          Length = 1855

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 841 LLGKKVSTK 849
>XP_005247550
          Length = 1856

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 910 LLGKKVSTK 918
>XP_006713712
          Length = 1923

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 910 LLGKKVSTK 918
>XP_005247549
          Length = 1924

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LLVKKVSTK 9
           LL KKVSTK
Sbjct: 910 LLGKKVSTK 918
>NP_114119
          Length = 886

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLVKKVST 8
           LL+KKV+T
Sbjct: 604 LLIKKVAT 611
>XP_006713840
          Length = 817

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLVKKVST 8
           LL+KKV+T
Sbjct: 604 LLIKKVAT 611
>XP_006713839
          Length = 874

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLVKKVST 8
           LL+KKV+T
Sbjct: 604 LLIKKVAT 611
>NP_758424
          Length = 1642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    VKKVSTK 9
            VKKV+TK
Sbjct: 1311 VKKVATK 1317
>NP_061142
          Length = 1642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    VKKVSTK 9
            VKKV+TK
Sbjct: 1311 VKKVATK 1317
>NP_689914
          Length = 5058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    LVKKVSTK 9
            LVKKVS K
Sbjct: 2528 LVKKVSGK 2535
>NP_066990
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KKVSTK 9
           KKVSTK
Sbjct: 108 KKVSTK 113
>NP_001103444
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 155 LLIDKVST 162
>NP_001159681
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 399 LLIDKVST 406
>NP_001159680
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 399 LLIDKVST 406
>NP_009217
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 155 LLIDKVST 162
>XP_005268428
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 155 LLIDKVST 162
>XP_005268427
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 155 LLIDKVST 162
>XP_005268426
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 155 LLIDKVST 162
>XP_006714818
          Length = 1147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLVKKVST 8
           LL+ KVST
Sbjct: 399 LLIDKVST 406
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.126    0.283 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 284,371
Number of extensions: 380
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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