Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_003706                                                              17    3301
NP_001276978                                                           17    3301
XP_006714460                                                           17    3301
XP_006714459                                                           17    3301
XP_006710243                                                           17    3301
NP_149113                                                              17    4312
NP_057197                                                              16    5631
NP_001153780                                                           16    5631
NP_061849                                                              16    5631
NP_114121                                                              16    5631
XP_006721606                                                           16    5631
XP_006721605                                                           16    5631
XP_006721604                                                           16    5631
XP_005256733                                                           16    5631
XP_005266629                                                           16    5631
XP_005266626                                                           16    5631
XP_005266625                                                           16    5631
XP_005269040                                                           16    5631
NP_057427                                                              16    7355
NP_055707                                                              16    7355
NP_001273128                                                           16    7355
NP_001273123                                                           16    7355
NP_001273118                                                           16    7355
NP_001273117                                                           16    7355
NP_061754                                                              16    7355
NP_001177766                                                           15    9605
NP_001128512                                                           15    9605
NP_004073                                                              15    9605
NP_075408                                                              15    9605
NP_001128513                                                           15    9605
NP_001177765                                                           15    9605
NP_055950                                                              15    9605
XP_005250292                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LLSETINTE 9
            L SETINTE
Sbjct: 1562 LFSETINTE 1570
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    LLSETINTE 9
            L SETINTE
Sbjct: 1562 LFSETINTE 1570
>NP_003706
          Length = 962

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           +L+ETINT
Sbjct: 329 ILTETINT 336
>NP_001276978
          Length = 973

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           +L+ETINT
Sbjct: 333 ILTETINT 340
>XP_006714460
          Length = 966

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           +L+ETINT
Sbjct: 333 ILTETINT 340
>XP_006714459
          Length = 969

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           +L+ETINT
Sbjct: 329 ILTETINT 336
>XP_006710243
          Length = 98

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SETINTE 9
          SET+NTE
Sbjct: 88 SETLNTE 94
>NP_149113
          Length = 395

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LSETINTE 9
           LSET NTE
Sbjct: 343 LSETDNTE 350
>NP_057197
          Length = 764

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           L+SET+N+
Sbjct: 644 LMSETVNS 651
>NP_001153780
          Length = 707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LLSETINT 8
           LLSET NT
Sbjct: 603 LLSETKNT 610
>NP_061849
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           +++ETINT
Sbjct: 252 IINETINT 259
>NP_114121
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LLSETINT 8
           LLSET NT
Sbjct: 615 LLSETKNT 622
>XP_006721606
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           L+SET+N+
Sbjct: 644 LMSETVNS 651
>XP_006721605
          Length = 680

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           L+SET+N+
Sbjct: 644 LMSETVNS 651
>XP_006721604
          Length = 733

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           L+SET+N+
Sbjct: 644 LMSETVNS 651
>XP_005256733
          Length = 763

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           L+SET+N+
Sbjct: 643 LMSETVNS 650
>XP_005266629
          Length = 518

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LLSETINT 8
           LLSET NT
Sbjct: 414 LLSETKNT 421
>XP_005266626
          Length = 695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LLSETINT 8
           LLSET NT
Sbjct: 591 LLSETKNT 598
>XP_005266625
          Length = 707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   LLSETINT 8
           LLSET NT
Sbjct: 603 LLSETKNT 610
>XP_005269040
          Length = 406

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLSETINT 8
           +++ETINT
Sbjct: 152 IINETINT 159
>NP_057427
          Length = 3114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLSETINTE 9
           LLSET++ E
Sbjct: 946 LLSETLSLE 954
>NP_055707
          Length = 1271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ETINTE 9
           ET+NTE
Sbjct: 593 ETVNTE 598
>NP_001273128
          Length = 1271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ETINTE 9
           ET+NTE
Sbjct: 593 ETVNTE 598
>NP_001273123
          Length = 1316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ETINTE 9
           ET+NTE
Sbjct: 638 ETVNTE 643
>NP_001273118
          Length = 1337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ETINTE 9
           ET+NTE
Sbjct: 659 ETVNTE 664
>NP_001273117
          Length = 1349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ETINTE 9
           ET+NTE
Sbjct: 671 ETVNTE 676
>NP_061754
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  LLSETINTE 9
          LLSET++ E
Sbjct: 85 LLSETLDRE 93
>NP_001177766
          Length = 1271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSETINTE 9
           L ET+N E
Sbjct: 704 LDETVNVE 711
>NP_001128512
          Length = 1253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSETINTE 9
           L ET+N E
Sbjct: 691 LDETVNVE 698
>NP_004073
          Length = 1278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSETINTE 9
           L ET+N E
Sbjct: 711 LDETVNVE 718
>NP_075408
          Length = 1144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSETINTE 9
           L ET+N E
Sbjct: 577 LDETVNVE 584
>NP_001128513
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSETINTE 9
           L ET+N E
Sbjct: 577 LDETVNVE 584
>NP_001177765
          Length = 1236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSETINTE 9
           L ET+N E
Sbjct: 674 LDETVNVE 681
>NP_055950
          Length = 2012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LSETINTE 9
            LSET+N +
Sbjct: 1786 LSETVNRD 1793
>XP_005250292
          Length = 1654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LSETINTE 9
            LSET+N +
Sbjct: 1428 LSETVNRD 1435
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.118    0.270 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,065
Number of extensions: 518
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package