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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              18    1135
NP_001158184                                                           15    9605
NP_000472                                                              15    9605
NP_001158182                                                           15    9605
NP_001278295                                                           15    9605
NP_060250                                                              15    9605
NP_001158183                                                           15    9605
NP_067071                                                              15    9605
XP_006716523                                                           15    9605
XP_006716522                                                           15    9605
XP_005251324                                                           15    9605
XP_005251323                                                           15    9605

>NP_073713
          Length = 985

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LQAGVALT 9
           LQAGVALT
Sbjct: 457 LQAGVALT 464



>NP_001158184
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LLQAGVA 7
           +LQAGVA
Sbjct: 190 VLQAGVA 196



>NP_000472
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LLQAGVA 7
           +LQAGVA
Sbjct: 190 VLQAGVA 196



>NP_001158182
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LLQAGVA 7
           +LQAGVA
Sbjct: 146 VLQAGVA 152



>NP_001278295
          Length = 790

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LLQAGVA 7
          LLQAG+A
Sbjct: 27 LLQAGLA 33



>NP_060250
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LLQAGVALT 9
            LLQAG++ T
Sbjct: 2484 LLQAGLSRT 2492



>NP_001158183
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LLQAGVA 7
           +LQAGVA
Sbjct: 134 VLQAGVA 140



>NP_067071
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LQAGVALT 9
          L+AGVAL+
Sbjct: 10 LRAGVALS 17



>XP_006716523
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LLQAGVALT 9
            LLQAG++ T
Sbjct: 1399 LLQAGLSRT 1407



>XP_006716522
          Length = 2192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LLQAGVALT 9
            LLQAG++ T
Sbjct: 1679 LLQAGLSRT 1687



>XP_005251324
          Length = 2996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LLQAGVALT 9
            LLQAG++ T
Sbjct: 2483 LLQAGLSRT 2491



>XP_005251323
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    LLQAGVALT 9
            LLQAG++ T
Sbjct: 2484 LLQAGLSRT 2492



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.136    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 339,432
Number of extensions: 294
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)