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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     509
NP_004561                                                              20     509
NP_001275703                                                           20     509
XP_005253450                                                           20     509
XP_006719151                                                           20     509
NP_001004692                                                           16    5631
NP_001004695                                                           16    5631
NP_001175                                                              15    9605
NP_001274152                                                           15    9605
NP_054858                                                              15    9605

>NP_001275701
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLNLMEMML 9
            LLNL+EMML
Sbjct: 1154 LLNLLEMML 1162



>NP_004561
          Length = 1445

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLNLMEMML 9
            LLNL+EMML
Sbjct: 1113 LLNLLEMML 1121



>NP_001275703
          Length = 1264

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LLNLMEMML 9
           LLNL+EMML
Sbjct: 932 LLNLLEMML 940



>XP_005253450
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLNLMEMML 9
            LLNL+EMML
Sbjct: 1154 LLNLLEMML 1162



>XP_006719151
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLNLMEMML 9
            LLNL+EMML
Sbjct: 1154 LLNLLEMML 1162



>NP_001004692
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LNLMEMML 9
          L+LM+MML
Sbjct: 64 LSLMDMML 71



>NP_001004695
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LNLMEMML 9
          L+LM+MML
Sbjct: 64 LSLMDMML 71



>NP_001175
          Length = 2644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LLNLME 6
           LLNLME
Sbjct: 809 LLNLME 814



>NP_001274152
          Length = 1791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    LLNLMEM 7
            L+NLME+
Sbjct: 1137 LINLMEL 1143



>NP_054858
          Length = 1791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    LLNLMEM 7
            L+NLME+
Sbjct: 1137 LINLMEL 1143



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.148    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,280
Number of extensions: 481
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)