Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     509
NP_004561                                                              20     509
NP_001275703                                                           20     509
XP_005253450                                                           20     509
XP_006719151                                                           20     509
NP_005488                                                              17    4312
XP_005256978                                                           17    4312
XP_005256977                                                           17    4312
NP_001073852                                                           17    4312
NP_060264                                                              16    5631
XP_005251551                                                           16    5631
XP_006716857                                                           16    5631
NP_001186704                                                           16    7355
NP_001295                                                              16    7355
NP_477352                                                              16    7355
NP_060392                                                              16    7355
NP_775901                                                              16    7355
NP_002051                                                              16    7355
XP_005261692                                                           16    7355
XP_005261691                                                           16    7355
XP_005266736                                                           16    7355
XP_006722498                                                           16    7355
XP_006722497                                                           16    7355
XP_005270807                                                           16    7355
NP_001175                                                              15    9605
NP_001274152                                                           15    9605
NP_689681                                                              15    9605
NP_777582                                                              15    9605
NP_054858                                                              15    9605
XP_006721789                                                           15    9605
XP_006716590                                                           15    9605
XP_006716589                                                           15    9605
>NP_001275701
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLLNLMEMM 9
            LLLNL+EMM
Sbjct: 1153 LLLNLLEMM 1161
>NP_004561
          Length = 1445

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLLNLMEMM 9
            LLLNL+EMM
Sbjct: 1112 LLLNLLEMM 1120
>NP_001275703
          Length = 1264

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LLLNLMEMM 9
           LLLNL+EMM
Sbjct: 931 LLLNLLEMM 939
>XP_005253450
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLLNLMEMM 9
            LLLNL+EMM
Sbjct: 1153 LLLNLLEMM 1161
>XP_006719151
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LLLNLMEMM 9
            LLLNL+EMM
Sbjct: 1153 LLLNLLEMM 1161
>NP_005488
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLN+ EM+
Sbjct: 243 LLLNIWEML 251
>XP_005256978
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLN+ EM+
Sbjct: 243 LLLNIWEML 251
>XP_005256977
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLN+ EM+
Sbjct: 243 LLLNIWEML 251
>NP_001073852
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLN+ EM+
Sbjct: 243 LLLNIWEML 251
>NP_060264
          Length = 1801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           L LNL+EM+
Sbjct: 380 LALNLLEMI 388
>XP_005251551
          Length = 1801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           L LNL+EM+
Sbjct: 380 LALNLLEMI 388
>XP_006716857
          Length = 1834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           L LNL+EM+
Sbjct: 413 LALNLLEMI 421
>NP_001186704
          Length = 1133

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLNL+E +
Sbjct: 326 LLLNLIEYL 334
>NP_001295
          Length = 1380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLNL+E +
Sbjct: 573 LLLNLIEYL 581
>NP_477352
          Length = 2102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLLNLMEM 8
           LL+NL+E+
Sbjct: 729 LLMNLLEL 736
>NP_060392
          Length = 669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLLNLMEM 8
           L LNL+EM
Sbjct: 529 LYLNLLEM 536
>NP_775901
          Length = 2226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LLLNLMEM 8
            LLLN ME+
Sbjct: 1218 LLLNFMEV 1225
>NP_002051
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLLNLMEMM 9
           L+LNL+E++
Sbjct: 221 LVLNLLELV 229
>XP_005261692
          Length = 1854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLLNLMEM 8
           LL+NL+E+
Sbjct: 757 LLMNLLEL 764
>XP_005261691
          Length = 2099

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LLLNLMEM 8
           LL+NL+E+
Sbjct: 757 LLMNLLEL 764
>XP_005266736
          Length = 1314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LLLNLMEM 8
           LLLN ME+
Sbjct: 306 LLLNFMEV 313
>XP_006722498
          Length = 2059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LLLNLMEM 8
            LLLN ME+
Sbjct: 1219 LLLNFMEV 1226
>XP_006722497
          Length = 2227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    LLLNLMEM 8
            LLLN ME+
Sbjct: 1219 LLLNFMEV 1226
>XP_005270807
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LLLNLMEMM 9
           L+LNL+E++
Sbjct: 221 LVLNLLELV 229
>NP_001175
          Length = 2644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LLNLME 7
           LLNLME
Sbjct: 809 LLNLME 814
>NP_001274152
          Length = 1791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    LLNLMEM 8
            L+NLME+
Sbjct: 1137 LINLMEL 1143
>NP_689681
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLNL+ ++
Sbjct: 117 LLLNLLSLL 125
>NP_777582
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLL L+E+M
Sbjct: 247 LLLRLVEVM 255
>NP_054858
          Length = 1791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    LLNLMEM 8
            L+NLME+
Sbjct: 1137 LINLMEL 1143
>XP_006721789
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLLNL+ ++
Sbjct: 117 LLLNLLSLL 125
>XP_006716590
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLL L+E+M
Sbjct: 140 LLLRLVEVM 148
>XP_006716589
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LLLNLMEMM 9
           LLL L+E+M
Sbjct: 247 LLLRLVEVM 255
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.148    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 356,282
Number of extensions: 561
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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