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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1935
NP_060862                                                              18    1935
XP_005263172                                                           18    1935
NP_036270                                                              17    2528
NP_001074003                                                           17    3301
XP_005254452                                                           17    3301
XP_005254451                                                           17    3301
NP_115548                                                              16    5631
XP_005249939                                                           16    5631
XP_006715854                                                           16    5631
XP_006715853                                                           16    5631
NP_000693                                                              16    7355
NP_065974                                                              16    7355
NP_001272478                                                           16    7355
XP_006712720                                                           16    7355
XP_006712719                                                           16    7355
XP_005246770                                                           16    7355
XP_005246768                                                           16    7355
XP_005246769                                                           16    7355
NP_001273001                                                           15    9605
NP_001123551                                                           15    9605
NP_612431                                                              15    9605
NP_001138573                                                           15    9605
NP_066014                                                              15    9605
XP_006718727                                                           15    9605
XP_006716107                                                           15    9605
XP_005270926                                                           15    9605
XP_005270927                                                           15    9605
XP_005270925                                                           15    9605

>NP_004295
          Length = 1620

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKVGSVRKL 9
           LK GSVRKL
Sbjct: 127 LKGGSVRKL 135



>NP_060862
          Length = 2104

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    LKVGSVRKL 9
            ++VGSVRK+
Sbjct: 1717 IRVGSVRKI 1725



>XP_005263172
          Length = 2104

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    LKVGSVRKL 9
            ++VGSVRK+
Sbjct: 1717 IRVGSVRKI 1725



>NP_036270
          Length = 560

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  LKVGSVRKL 9
          L VGS+RKL
Sbjct: 51 LVVGSIRKL 59



>NP_001074003
          Length = 2214

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LKVGSVRKL 9
           LK+G++RKL
Sbjct: 176 LKLGALRKL 184



>XP_005254452
          Length = 2188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LKVGSVRKL 9
           LK+G++RKL
Sbjct: 176 LKLGALRKL 184



>XP_005254451
          Length = 2214

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LKVGSVRKL 9
           LK+G++RKL
Sbjct: 176 LKLGALRKL 184



>NP_115548
          Length = 1316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KVGSVRKL 9
           K+GS+RK+
Sbjct: 941 KIGSLRKV 948



>XP_005249939
          Length = 1324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KVGSVRKL 9
           K+GS+RK+
Sbjct: 941 KIGSLRKV 948



>XP_006715854
          Length = 1327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KVGSVRKL 9
           K+GS+RK+
Sbjct: 941 KIGSLRKV 948



>XP_006715853
          Length = 1327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   KVGSVRKL 9
           K+GS+RK+
Sbjct: 941 KIGSLRKV 948



>NP_000693
          Length = 1020

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKVGSVRKL 9
           L  GSVRK+
Sbjct: 460 LSCGSVRKM 468



>NP_065974
          Length = 2354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1793 LKVDSVRNL 1801



>NP_001272478
          Length = 2352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1791 LKVDSVRNL 1799



>XP_006712720
          Length = 1991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1793 LKVDSVRNL 1801



>XP_006712719
          Length = 2354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1793 LKVDSVRNL 1801



>XP_005246770
          Length = 2354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1793 LKVDSVRNL 1801



>XP_005246768
          Length = 2354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1793 LKVDSVRNL 1801



>XP_005246769
          Length = 2354

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKVGSVRKL 9
            LKV SVR L
Sbjct: 1793 LKVDSVRNL 1801



>NP_001273001
          Length = 947

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LKVGSVRK 8
           LKVGSV +
Sbjct: 804 LKVGSVEE 811



>NP_001123551
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LKVGSVRK 8
           LKVGSV +
Sbjct: 860 LKVGSVEE 867



>NP_612431
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LKVGSVRKL 9
           ++ G+VRKL
Sbjct: 276 VRAGAVRKL 284



>NP_001138573
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LKVGSVRKL 9
          LK+ ++RKL
Sbjct: 81 LKISTLRKL 89



>NP_066014
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LKVGSVRK 8
           LKVGSV +
Sbjct: 860 LKVGSVEE 867



>XP_006718727
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LKVGSVRKL 9
          LK+ ++RKL
Sbjct: 81 LKISTLRKL 89



>XP_006716107
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KVGSVRK 8
           K+GSVR+
Sbjct: 566 KIGSVRE 572



>XP_005270926
          Length = 947

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LKVGSVRK 8
           LKVGSV +
Sbjct: 804 LKVGSVEE 811



>XP_005270927
          Length = 947

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LKVGSVRK 8
           LKVGSV +
Sbjct: 804 LKVGSVEE 811



>XP_005270925
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   LKVGSVRK 8
           LKVGSV +
Sbjct: 860 LKVGSVEE 867



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.141    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 427,428
Number of extensions: 524
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)