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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_008949                                                              15    9605
NP_005081                                                              15    9605
NP_001185517                                                           15    9605
XP_006717006                                                           15    9605
XP_006717005                                                           15    9605
XP_006717004                                                           15    9605
XP_006717003                                                           15    9605
XP_005251736                                                           15    9605
NP_001108105                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LKTQVTSAV 9
           LKTQ+TSAV
Sbjct: 204 LKTQMTSAV 212



>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LKTQVTSAV 9
           LKTQ+TSAV
Sbjct: 204 LKTQMTSAV 212



>NP_008949
          Length = 2325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LKTQVTS 7
            LK+QVTS
Sbjct: 1585 LKSQVTS 1591



>NP_005081
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LKTQVT 6
           LKTQVT
Sbjct: 593 LKTQVT 598



>NP_001185517
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LKTQVT 6
           LKTQVT
Sbjct: 593 LKTQVT 598



>XP_006717006
          Length = 1760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LKTQVTS 7
            LK+QVTS
Sbjct: 1005 LKSQVTS 1011



>XP_006717005
          Length = 1788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LKTQVTS 7
            LK+QVTS
Sbjct: 1033 LKSQVTS 1039



>XP_006717004
          Length = 2240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LKTQVTS 7
            LK+QVTS
Sbjct: 1585 LKSQVTS 1591



>XP_006717003
          Length = 2278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LKTQVTS 7
            LK+QVTS
Sbjct: 1585 LKSQVTS 1591



>XP_005251736
          Length = 2325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LKTQVTS 7
            LK+QVTS
Sbjct: 1585 LKSQVTS 1591



>NP_001108105
          Length = 781

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LKTQVT 6
           LKTQVT
Sbjct: 362 LKTQVT 367



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.110    0.244 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 248,674
Number of extensions: 180
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)