Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              19     869
NP_733762                                                              19     869
XP_006713860                                                           19     869
XP_006713859                                                           19     869
XP_005247910                                                           19     869
XP_005247909                                                           19     869
NP_001034568                                                           17    2528
XP_005268301                                                           17    2528
XP_006720407                                                           17    2528
NP_057313                                                              17    3301
XP_006718315                                                           17    3301
XP_005253032                                                           17    3301
XP_006718314                                                           17    3301
XP_005253031                                                           17    3301
XP_006718313                                                           17    3301
XP_005253030                                                           17    3301
NP_085143                                                              17    4312
XP_006719218                                                           17    4312
NP_000724                                                              16    5631
NP_057092                                                              16    5631
NP_056337                                                              16    5631
XP_005264422                                                           16    5631
XP_005264421                                                           16    5631
NP_001180393                                                           16    5631
NP_061921                                                              16    5631
NP_006436                                                              16    7355
NP_660339                                                              16    7355
NP_065744                                                              16    7355
NP_112536                                                              16    7355
NP_001273660                                                           16    7355
NP_997722                                                              16    7355
XP_005258677                                                           16    7355
XP_005246710                                                           16    7355
NP_001106881                                                           16    7355
NP_002776                                                              16    7355
NP_976032                                                              16    7355
NP_006197                                                              15    9605
NP_000307                                                              15    9605
NP_001171673                                                           15    9605
NP_055598                                                              15    9605
NP_955389                                                              15    9605
XP_006725628                                                           15    9605
XP_006725627                                                           15    9605
XP_005254865                                                           15    9605
XP_006714104                                                           15    9605
XP_005265800                                                           15    9605
XP_006714103                                                           15    9605
XP_006714102                                                           15    9605
XP_005265401                                                           15    9605
XP_005265400                                                           15    9605
XP_005265399                                                           15    9605
XP_006713335                                                           15    9605
>NP_005179
          Length = 906

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKSTIVLTC 9
           LKSTI LTC
Sbjct: 224 LKSTIDLTC 232
>NP_733762
          Length = 982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKSTIVLTC 9
           LKSTI LTC
Sbjct: 216 LKSTIDLTC 224
>XP_006713860
          Length = 914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKSTIVLTC 9
           LKSTI LTC
Sbjct: 244 LKSTIDLTC 252
>XP_006713859
          Length = 966

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKSTIVLTC 9
           LKSTI LTC
Sbjct: 244 LKSTIDLTC 252
>XP_005247910
          Length = 982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKSTIVLTC 9
           LKSTI LTC
Sbjct: 216 LKSTIDLTC 224
>XP_005247909
          Length = 1010

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKSTIVLTC 9
           LKSTI LTC
Sbjct: 244 LKSTIDLTC 252
>NP_001034568
          Length = 823

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   STIVLTC 9
           ST+VLTC
Sbjct: 175 STLVLTC 181
>XP_005268301
          Length = 662

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  STIVLTC 9
          ST+VLTC
Sbjct: 14 STLVLTC 20
>XP_006720407
          Length = 813

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   STIVLTC 9
           ST+VLTC
Sbjct: 165 STLVLTC 171
>NP_057313
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 242 KSTLILVC 249
>XP_006718315
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 242 KSTLILVC 249
>XP_005253032
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 242 KSTLILVC 249
>XP_006718314
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 242 KSTLILVC 249
>XP_005253031
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 242 KSTLILVC 249
>XP_006718313
          Length = 276

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 242 KSTLILVC 249
>XP_005253030
          Length = 336

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST++L C
Sbjct: 302 KSTLILVC 309
>NP_085143
          Length = 665

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST+V TC
Sbjct: 139 KSTLVPTC 146
>XP_006719218
          Length = 665

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KSTIVLTC 9
           KST+V TC
Sbjct: 139 KSTLVPTC 146
>NP_000724
          Length = 207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  STIVLTC 9
          +T++LTC
Sbjct: 43 TTVILTC 49
>NP_057092
          Length = 351

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KSTIVLTC 9
          K+TI+L C
Sbjct: 44 KTTIILRC 51
>NP_056337
          Length = 201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KSTIVLTC 9
          K+TI+L C
Sbjct: 44 KTTIILRC 51
>XP_005264422
          Length = 373

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KSTIVLTC 9
          K+TI+L C
Sbjct: 44 KTTIILRC 51
>XP_005264421
          Length = 374

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KSTIVLTC 9
          K+TI+L C
Sbjct: 44 KTTIILRC 51
>NP_001180393
          Length = 352

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KSTIVLTC 9
          K+TI+L C
Sbjct: 44 KTTIILRC 51
>NP_061921
          Length = 643

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKSTIVLTC 9
           LKST++  C
Sbjct: 183 LKSTVISRC 191
>NP_006436
          Length = 2335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2    KSTIVLTC 9
            + TI++TC
Sbjct: 2209 EKTIIITC 2216
>NP_660339
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           T+VLTC
Sbjct: 240 TLVLTC 245
>NP_065744
          Length = 2113

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   STIVLTC 9
           ST++L+C
Sbjct: 390 STVILSC 396
>NP_112536
          Length = 335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           T++LTC
Sbjct: 164 TVILTC 169
>NP_001273660
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1 LKSTIVLTC 9
         ++STI+L C
Sbjct: 1 MRSTILLFC 9
>NP_997722
          Length = 209

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1 LKSTIVLTC 9
         ++STI+L C
Sbjct: 1 MRSTILLFC 9
>XP_005258677
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           T+VLTC
Sbjct: 240 TLVLTC 245
>XP_005246710
          Length = 643

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKSTIVLTC 9
           LKST++  C
Sbjct: 183 LKSTVISGC 191
>NP_001106881
          Length = 213

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4  TIVLTC 9
          T++LTC
Sbjct: 42 TVILTC 47
>NP_002776
          Length = 335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           T++LTC
Sbjct: 164 TVILTC 169
>NP_976032
          Length = 213

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4  TIVLTC 9
          T++LTC
Sbjct: 42 TVILTC 47
>NP_006197
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           TIV+TC
Sbjct: 230 TIVVTC 235
>NP_000307
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSTIVL 7
           LKST+VL
Sbjct: 407 LKSTLVL 413
>NP_001171673
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSTIVL 7
           LKST+VL
Sbjct: 407 LKSTLVL 413
>NP_055598
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KSTIVLTC 9
           K+T++L+C
Sbjct: 154 KATLLLSC 161
>NP_955389
          Length = 501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KSTIVLTC 9
           K+T++L+C
Sbjct: 154 KATLLLSC 161
>XP_006725628
          Length = 994

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KSTIVLTC 9
           K+T++L+C
Sbjct: 154 KATLLLSC 161
>XP_006725627
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KSTIVLTC 9
           K+T++L+C
Sbjct: 154 KATLLLSC 161
>XP_005254865
          Length = 994

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   KSTIVLTC 9
           K+T++L+C
Sbjct: 154 KATLLLSC 161
>XP_006714104
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           TIV+TC
Sbjct: 255 TIVVTC 260
>XP_005265800
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           TIV+TC
Sbjct: 230 TIVVTC 235
>XP_006714103
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           TIV+TC
Sbjct: 252 TIVVTC 257
>XP_006714102
          Length = 1114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TIVLTC 9
           TIV+TC
Sbjct: 255 TIVVTC 260
>XP_005265401
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSTIVL 7
           LKST+VL
Sbjct: 376 LKSTLVL 382
>XP_005265400
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSTIVL 7
           LKST+VL
Sbjct: 389 LKSTLVL 395
>XP_005265399
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSTIVL 7
           LKST+VL
Sbjct: 407 LKSTLVL 413
>XP_006713335
          Length = 606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   LKSTIVL 7
           LKST+VL
Sbjct: 420 LKSTLVL 426
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.135    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 384,200
Number of extensions: 567
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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