Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001138502                                                           17    2528
NP_006026                                                              17    3301
XP_005268287                                                           17    3301
XP_005268286                                                           17    3301
XP_005268285                                                           17    3301
XP_005268284                                                           17    3301
NP_444256                                                              17    4312
NP_444254                                                              17    4312
NP_444253                                                              17    4312
NP_444255                                                              17    4312
NP_056175                                                              17    4312
XP_005247551                                                           17    4312
XP_005247550                                                           17    4312
XP_006713712                                                           17    4312
XP_005247549                                                           17    4312
NP_004475                                                              16    5631
NP_001158089                                                           16    5631
NP_001010888                                                           16    7355
NP_001273290                                                           16    7355
NP_001273289                                                           16    7355
NP_001157787                                                           16    7355
NP_079466                                                              16    7355
XP_003960344                                                           16    7355
XP_005262317                                                           16    7355
XP_006712951                                                           16    7355
XP_006712731                                                           16    7355
XP_006712730                                                           16    7355
XP_006712729                                                           16    7355
XP_006712728                                                           16    7355
XP_006712727                                                           16    7355
XP_006712726                                                           16    7355
XP_006712725                                                           16    7355
XP_006712724                                                           16    7355
XP_005264052                                                           16    7355
XP_005263995                                                           16    7355
XP_006712580                                                           16    7355
XP_005263994                                                           16    7355
XP_006712579                                                           16    7355
XP_006712578                                                           16    7355
XP_006712577                                                           16    7355
XP_006710197                                                           16    7355
NP_005870                                                              16    7355
NP_005723                                                              15    9605
NP_955374                                                              15    9605
NP_037490                                                              15    9605
>NP_001138502
          Length = 1692

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   LKSASKELK 9
           L+SAS+ELK
Sbjct: 471 LRSASEELK 479
>NP_006026
          Length = 1711

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           LKS +KELK
Sbjct: 556 LKSQAKELK 564
>XP_005268287
          Length = 1490

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           LKS +KELK
Sbjct: 556 LKSQAKELK 564
>XP_005268286
          Length = 1685

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           LKS +KELK
Sbjct: 556 LKSQAKELK 564
>XP_005268285
          Length = 1702

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           LKS +KELK
Sbjct: 556 LKSQAKELK 564
>XP_005268284
          Length = 1728

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           LKS +KELK
Sbjct: 556 LKSQAKELK 564
>NP_444256
          Length = 1794

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LKSASKE 7
           LKSASKE
Sbjct: 990 LKSASKE 996
>NP_444254
          Length = 1845

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   LKSASKE 7
           LKSASKE
Sbjct: 990 LKSASKE 996
>NP_444253
          Length = 1914

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    LKSASKE 7
            LKSASKE
Sbjct: 1059 LKSASKE 1065
>NP_444255
          Length = 1863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    LKSASKE 7
            LKSASKE
Sbjct: 1059 LKSASKE 1065
>NP_056175
          Length = 1042

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           +KSA +ELK
Sbjct: 832 IKSAKRELK 840
>XP_005247551
          Length = 1855

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    LKSASKE 7
            LKSASKE
Sbjct: 1000 LKSASKE 1006
>XP_005247550
          Length = 1856

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    LKSASKE 7
            LKSASKE
Sbjct: 1069 LKSASKE 1075
>XP_006713712
          Length = 1923

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    LKSASKE 7
            LKSASKE
Sbjct: 1069 LKSASKE 1075
>XP_005247549
          Length = 1924

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    LKSASKE 7
            LKSASKE
Sbjct: 1069 LKSASKE 1075
>NP_004475
          Length = 580

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           L+SAS ELK
Sbjct: 93  LQSASMELK 101
>NP_001158089
          Length = 603

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           L+SAS ELK
Sbjct: 93  LQSASMELK 101
>NP_001010888
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  KSASKELK 9
          KSASKE K
Sbjct: 14 KSASKEEK 21
>NP_001273290
          Length = 199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  KSASKELK 9
          KSA++ELK
Sbjct: 41 KSAAEELK 48
>NP_001273289
          Length = 279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  KSASKELK 9
          KSA++ELK
Sbjct: 41 KSAAEELK 48
>NP_001157787
          Length = 1941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1507 LKSAEVELK 1515
>NP_079466
          Length = 1353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 919 LKSAEVELK 927
>XP_003960344
          Length = 2085

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1651 LKSAEVELK 1659
>XP_005262317
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  KSASKELK 9
          KSASKE K
Sbjct: 14 KSASKEEK 21
>XP_006712951
          Length = 2119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1685 LKSAEVELK 1693
>XP_006712731
          Length = 1102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 668 LKSAEVELK 676
>XP_006712730
          Length = 1106

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 672 LKSAEVELK 680
>XP_006712729
          Length = 1121

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 687 LKSAEVELK 695
>XP_006712728
          Length = 1307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 873 LKSAEVELK 881
>XP_006712727
          Length = 1319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 885 LKSAEVELK 893
>XP_006712726
          Length = 1319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 885 LKSAEVELK 893
>XP_006712725
          Length = 1352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 918 LKSAEVELK 926
>XP_006712724
          Length = 1353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 919 LKSAEVELK 927
>XP_005264052
          Length = 1353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 919 LKSAEVELK 927
>XP_005263995
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 948 LKSAEVELK 956
>XP_006712580
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKSA  ELK
Sbjct: 948 LKSAEVELK 956
>XP_005263994
          Length = 1496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1062 LKSAEVELK 1070
>XP_006712579
          Length = 1865

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1480 LKSAEVELK 1488
>XP_006712578
          Length = 1914

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1480 LKSAEVELK 1488
>XP_006712577
          Length = 1915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1481 LKSAEVELK 1489
>XP_006710197
          Length = 1836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LKSASKELK 9
            LKSA  ELK
Sbjct: 1402 LKSAEVELK 1410
>NP_005870
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LKSASKELK 9
           LKSA K+L+
Sbjct: 250 LKSAQKDLQ 258
>NP_005723
          Length = 1312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKSASKELK 9
           LKS + ELK
Sbjct: 863 LKSTTNELK 871
>NP_955374
          Length = 66

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  KSASKELK 9
          KSA +ELK
Sbjct: 25 KSAQQELK 32
>NP_037490
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SASKELK 9
          +ASKELK
Sbjct: 82 TASKELK 88
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.107    0.227 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 372,499
Number of extensions: 364
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)

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