Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_001036065                                                           17    3301
NP_009112                                                              17    3301
XP_006723056                                                           17    3301
NP_055935                                                              17    4312
NP_006292                                                              17    4312
NP_001180440                                                           17    4312
XP_006719652                                                           17    4312
XP_006719651                                                           17    4312
XP_005269195                                                           17    4312
NP_079321                                                              16    5631
NP_597721                                                              16    5631
NP_001007170                                                           16    5631
NP_005099                                                              16    5631
XP_005261808                                                           16    5631
XP_006713512                                                           16    5631
XP_006713511                                                           16    5631
NP_068741                                                              16    7355
NP_002344                                                              16    7355
NP_997304                                                              16    7355
XP_005248945                                                           16    7355
XP_005248944                                                           16    7355
XP_005248943                                                           16    7355
XP_005248942                                                           16    7355
NP_001128472                                                           15    9605
NP_004658                                                              15    9605
NP_001128471                                                           15    9605
NP_006823                                                              15    9605
NP_002829                                                              15    9605
NP_036271                                                              15    9605
NP_061740                                                              15    9605
NP_114442                                                              15    9605
XP_006720790                                                           15    9605
XP_005268334                                                           15    9605
XP_005268333                                                           15    9605
XP_006720789                                                           15    9605
XP_005267342                                                           15    9605
XP_006720073                                                           15    9605
XP_006720072                                                           15    9605
XP_006720071                                                           15    9605
XP_005246783                                                           15    9605
XP_006711535                                                           15    9605
NP_065994                                                              15    9605
>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKMTSMSPD 9
            L+MTSMSPD
Sbjct: 2563 LEMTSMSPD 2571
>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    LKMTSMSPD 9
            L+MTSMSPD
Sbjct: 2563 LEMTSMSPD 2571
>NP_001036065
          Length = 821

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LKMTSMSPD 9
          LKM  M PD
Sbjct: 45 LKMVKMEPD 53
>NP_009112
          Length = 833

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LKMTSMSPD 9
          LKM  M PD
Sbjct: 45 LKMVKMEPD 53
>XP_006723056
          Length = 801

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LKMTSMSPD 9
          LKM  M PD
Sbjct: 45 LKMVKMEPD 53
>NP_055935
          Length = 4169

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           L+M  MSPD
Sbjct: 459 LRMLRMSPD 467
>NP_006292
          Length = 859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMTSMSPD 9
           KM+S SPD
Sbjct: 605 KMSSSSPD 612
>NP_001180440
          Length = 892

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMTSMSPD 9
           KM+S SPD
Sbjct: 638 KMSSSSPD 645
>XP_006719652
          Length = 759

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMTSMSPD 9
           KM+S SPD
Sbjct: 505 KMSSSSPD 512
>XP_006719651
          Length = 892

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMTSMSPD 9
           KM+S SPD
Sbjct: 638 KMSSSSPD 645
>XP_005269195
          Length = 892

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KMTSMSPD 9
           KM+S SPD
Sbjct: 638 KMSSSSPD 645
>NP_079321
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSMSP 8
           MTSMSP
Sbjct: 402 MTSMSP 407
>NP_597721
          Length = 744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KMTSMSPD 9
          KMT++SP+
Sbjct: 9  KMTAISPE 16
>NP_001007170
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KMTSMSPD 9
          KMT++SP+
Sbjct: 9  KMTAISPE 16
>NP_005099
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTSMSPD 9
           +TS+SPD
Sbjct: 112 LTSLSPD 118
>XP_005261808
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   MTSMSP 8
           MTSMSP
Sbjct: 402 MTSMSP 407
>XP_006713512
          Length = 461

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MTSMSPD 9
           +TS+SPD
Sbjct: 112 LTSLSPD 118
>XP_006713511
          Length = 509

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  MTSMSPD 9
          +TS+SPD
Sbjct: 85 LTSLSPD 91
>NP_068741
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           +KM S+ PD
Sbjct: 424 VKMESLEPD 432
>NP_002344
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KMTSMSPD 9
          KMT  SPD
Sbjct: 40 KMTVCSPD 47
>NP_997304
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LKMTSMSP 8
          +KMTS SP
Sbjct: 85 VKMTSPSP 92
>XP_005248945
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           +KM S+ PD
Sbjct: 424 VKMESLEPD 432
>XP_005248944
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           +KM S+ PD
Sbjct: 424 VKMESLEPD 432
>XP_005248943
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           +KM S+ PD
Sbjct: 401 VKMESLEPD 409
>XP_005248942
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           +KM S+ PD
Sbjct: 417 VKMESLEPD 425
>NP_001128472
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 496 LKMQHLNPD 504
>NP_004658
          Length = 4834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KMTSMSPD 9
            KM+S  PD
Sbjct: 4450 KMSSFGPD 4457
>NP_001128471
          Length = 687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 496 LKMQHLNPD 504
>NP_006823
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 496 LKMQHLNPD 504
>NP_002829
          Length = 1306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  TSMSPD 9
          TS+SPD
Sbjct: 74 TSLSPD 79
>NP_036271
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KMTSMSPD 9
           K+T +SPD
Sbjct: 413 KVTYLSPD 420
>NP_061740
          Length = 962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   LKMTSMSPD 9
           L MT+  PD
Sbjct: 503 LSMTAQDPD 511
>NP_114442
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   LKMTSMSPD 9
           L MT+  PD
Sbjct: 503 LSMTAQDPD 511
>XP_006720790
          Length = 4748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KMTSMSPD 9
            KM+S  PD
Sbjct: 4364 KMSSFGPD 4371
>XP_005268334
          Length = 4796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KMTSMSPD 9
            KM+S  PD
Sbjct: 4412 KMSSFGPD 4419
>XP_005268333
          Length = 4796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KMTSMSPD 9
            KM+S  PD
Sbjct: 4412 KMSSFGPD 4419
>XP_006720789
          Length = 4829

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    KMTSMSPD 9
            KM+S  PD
Sbjct: 4445 KMSSFGPD 4452
>XP_005267342
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 507 LKMQHLNPD 515
>XP_006720073
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 496 LKMQHLNPD 504
>XP_006720072
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 507 LKMQHLNPD 515
>XP_006720071
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LKMTSMSPD 9
           LKM  ++PD
Sbjct: 507 LKMQHLNPD 515
>XP_005246783
          Length = 908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKMTSMSP 8
           LK+T +SP
Sbjct: 302 LKLTQVSP 309
>XP_006711535
          Length = 1258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  TSMSPD 9
          TS+SPD
Sbjct: 74 TSLSPD 79
>NP_065994
          Length = 908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LKMTSMSP 8
           LK+T +SP
Sbjct: 302 LKLTQVSP 309
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.115    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 475,523
Number of extensions: 754
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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