Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              18    1935
XP_006717990                                                           18    1935
XP_006717989                                                           18    1935
NP_110413                                                              17    4312
NP_001155180                                                           17    4312
NP_001157849                                                           16    5631
XP_005264064                                                           16    5631
XP_005264063                                                           16    5631
XP_005264062                                                           16    5631
XP_005264061                                                           16    5631
XP_005264059                                                           16    5631
NP_001104026                                                           16    7355
NP_001449                                                              16    7355
NP_001120959                                                           16    7355
NP_001447                                                              16    7355
NP_001073291                                                           16    7355
NP_000417                                                              16    7355
NP_001258779                                                           16    7355
NP_001275949                                                           16    7355
NP_659464                                                              16    7355
NP_954863                                                              16    7355
XP_006715973                                                           16    7355
XP_005267039                                                           16    7355
XP_005267038                                                           16    7355
XP_005248961                                                           16    7355
NP_570141                                                              16    7355
NP_001135440                                                           16    7355
NP_001182156                                                           15    9605
NP_060106                                                              15    9605
NP_001161147                                                           15    9605
NP_001161146                                                           15    9605
NP_694854                                                              15    9605
NP_000921                                                              15    9605
NP_127509                                                              15    9605
NP_067012                                                              15    9605
NP_001120645                                                           15    9605
NP_001164564                                                           15    9605
NP_653236                                                              15    9605
XP_005259075                                                           15    9605
XP_005259074                                                           15    9605
XP_006723312                                                           15    9605
XP_005247172                                                           15    9605
XP_005247171                                                           15    9605
XP_005247170                                                           15    9605
XP_006713145                                                           15    9605
XP_006713144                                                           15    9605
>NP_000305
          Length = 403

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKAQEALYF 9
           LKAQEAL F
Sbjct: 146 LKAQEALDF 154
>XP_006717990
          Length = 175

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKAQEALYF 9
           LKAQEAL F
Sbjct: 131 LKAQEALDF 139
>XP_006717989
          Length = 388

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LKAQEALYF 9
           LKAQEAL F
Sbjct: 131 LKAQEALDF 139
>NP_110413
          Length = 482

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KAQEALY 8
          KA+EALY
Sbjct: 78 KAEEALY 84
>NP_001155180
          Length = 476

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KAQEALY 8
          KA+EALY
Sbjct: 78 KAEEALY 84
>NP_001157849
          Length = 289

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LKAQEALYF 9
          L AQ+ LYF
Sbjct: 74 LGAQDTLYF 82
>XP_005264064
          Length = 2282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    KAQEALYF 9
            K QEA YF
Sbjct: 1809 KVQEAQYF 1816
>XP_005264063
          Length = 3136

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    KAQEALYF 9
            K QEA YF
Sbjct: 2663 KVQEAQYF 2670
>XP_005264062
          Length = 3232

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    KAQEALYF 9
            K QEA YF
Sbjct: 2759 KVQEAQYF 2766
>XP_005264061
          Length = 3232

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    KAQEALYF 9
            K QEA YF
Sbjct: 2759 KVQEAQYF 2766
>XP_005264059
          Length = 3258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    KAQEALYF 9
            K QEA YF
Sbjct: 2785 KVQEAQYF 2792
>NP_001104026
          Length = 2647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKAQEALYF 9
           LKA E  YF
Sbjct: 784 LKAHEPTYF 792
>NP_001449
          Length = 2725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKAQEALYF 9
           LKA E  YF
Sbjct: 779 LKANEPTYF 787
>NP_001120959
          Length = 2692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKAQEALYF 9
           LKA E  YF
Sbjct: 779 LKANEPTYF 787
>NP_001447
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKAQEALYF 9
           LKA E  YF
Sbjct: 784 LKAHEPTYF 792
>NP_001073291
          Length = 3118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LKAQEALYF 9
            LKA + +YF
Sbjct: 2462 LKADDKIYF 2470
>NP_000417
          Length = 3122

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LKAQEALYF 9
            LKA + +YF
Sbjct: 2462 LKADDKIYF 2470
>NP_001258779
          Length = 193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LKAQEALYF 9
          L A+E+LY+
Sbjct: 13 LTAEESLYY 21
>NP_001275949
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KAQEALY 8
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_659464
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KAQEALY 8
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_954863
          Length = 522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LKAQEALYF 9
           L A+E+LY+
Sbjct: 342 LTAEESLYY 350
>XP_006715973
          Length = 2632

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LKAQEALYF 9
           LKA E  YF
Sbjct: 779 LKANEPTYF 787
>XP_005267039
          Length = 3206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LKAQEALYF 9
            LKA + +YF
Sbjct: 2550 LKADDKIYF 2558
>XP_005267038
          Length = 3210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    LKAQEALYF 9
            LKA + +YF
Sbjct: 2550 LKADDKIYF 2558
>XP_005248961
          Length = 842

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KAQEALY 8
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_570141
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LKAQEALYF 9
           L A+E+LY+
Sbjct: 265 LTAEESLYY 273
>NP_001135440
          Length = 411

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   LKAQEALYF 9
           L A+E+LY+
Sbjct: 231 LTAEESLYY 239
>NP_001182156
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQEALYF 9
           +AQ A+YF
Sbjct: 571 RAQMAMYF 578
>NP_060106
          Length = 1214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQEALYF 9
           +AQ A+YF
Sbjct: 571 RAQMAMYF 578
>NP_001161147
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AQEALYF 9
          AQ+ LYF
Sbjct: 69 AQQTLYF 75
>NP_001161146
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AQEALYF 9
          AQ+ LYF
Sbjct: 60 AQQTLYF 66
>NP_694854
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AQEALYF 9
          AQ+ LYF
Sbjct: 62 AQQTLYF 68
>NP_000921
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   QEALYF 9
           Q+ALYF
Sbjct: 98  QQALYF 103
>NP_127509
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  QEALYF 9
          Q+ALYF
Sbjct: 52 QQALYF 57
>NP_067012
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AQEALYF 9
           AQ+ LYF
Sbjct: 100 AQQTLYF 106
>NP_001120645
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AQEALYF 9
          AQ+ LYF
Sbjct: 66 AQQTLYF 72
>NP_001164564
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LKAQEALY 8
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>NP_653236
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LKAQEALY 8
           L+AQ++LY
Sbjct: 299 LEAQDSLY 306
>XP_005259075
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KAQEALYF 9
          +AQ A+YF
Sbjct: 35 RAQMAMYF 42
>XP_005259074
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQEALYF 9
           +AQ A+YF
Sbjct: 142 RAQMAMYF 149
>XP_006723312
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQEALYF 9
           +AQ A+YF
Sbjct: 486 RAQMAMYF 493
>XP_005247172
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LKAQEALY 8
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>XP_005247171
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LKAQEALY 8
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>XP_005247170
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LKAQEALY 8
           L+AQ++LY
Sbjct: 214 LEAQDSLY 221
>XP_006713145
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AQEALYF 9
          AQ+ LYF
Sbjct: 60 AQQTLYF 66
>XP_006713144
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AQEALYF 9
          AQ+ LYF
Sbjct: 60 AQQTLYF 66
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.137    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 393,549
Number of extensions: 524
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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