Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              19     869
XP_005251512                                                           19     869
NP_619639                                                              16    5631
NP_001277199                                                           16    5631
NP_006857                                                              16    5631
NP_940851                                                              16    5631
NP_056532                                                              16    5631
NP_009224                                                              16    7355
NP_001239133                                                           16    7355
NP_001002029                                                           16    7355
NP_055918                                                              16    7355
NP_683692                                                              16    7355
NP_001229752                                                           16    7355
XP_005248208                                                           16    7355
XP_005248207                                                           16    7355
XP_006714021                                                           16    7355
NP_775099                                                              15    9605
NP_056472                                                              15    9605
NP_001274442                                                           15    9605
NP_003911                                                              15    9605
NP_056416                                                              15    9605
NP_001138392                                                           15    9605
NP_079202                                                              15    9605
NP_001274441                                                           15    9605
NP_001138395                                                           15    9605
NP_001265115                                                           15    9605
NP_001265114                                                           15    9605
XP_006717816                                                           15    9605
XP_006717815                                                           15    9605
XP_006717814                                                           15    9605
XP_005250665                                                           15    9605
XP_006716198                                                           15    9605
NP_071362                                                              15    9605
>NP_004963
          Length = 1132

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LIVNESLGQ 9
           LI NESLGQ
Sbjct: 545 LIFNESLGQ 553
>XP_005251512
          Length = 1132

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LIVNESLGQ 9
           LI NESLGQ
Sbjct: 545 LIFNESLGQ 553
>NP_619639
          Length = 4074

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    LIVNESLG 8
            L++NES+G
Sbjct: 3525 LLLNESIG 3532
>NP_001277199
          Length = 454

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LIVNESLGQ 9
           L+V+ SLGQ
Sbjct: 403 LVVSASLGQ 411
>NP_006857
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LIVNESLGQ 9
           L+V+ SLGQ
Sbjct: 415 LVVSASLGQ 423
>NP_940851
          Length = 327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NESLGQ 9
           NESLGQ
Sbjct: 322 NESLGQ 327
>NP_056532
          Length = 328

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IVNESLG 8
           +VNESLG
Sbjct: 111 MVNESLG 117
>NP_009224
          Length = 1744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           +NE LGQ
Sbjct: 687 INEKLGQ 693
>NP_001239133
          Length = 1698

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           +NE LGQ
Sbjct: 687 INEKLGQ 693
>NP_001002029
          Length = 1744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           +NE LGQ
Sbjct: 687 INEKLGQ 693
>NP_055918
          Length = 1925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    VNESLG 8
            VNESLG
Sbjct: 1236 VNESLG 1241
>NP_683692
          Length = 3051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LIVNESL 7
            L+VNESL
Sbjct: 2709 LLVNESL 2715
>NP_001229752
          Length = 1744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           +NE LGQ
Sbjct: 687 INEKLGQ 693
>XP_005248208
          Length = 3097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LIVNESL 7
            L+VNESL
Sbjct: 2709 LLVNESL 2715
>XP_005248207
          Length = 3098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LIVNESL 7
            L+VNESL
Sbjct: 2709 LLVNESL 2715
>XP_006714021
          Length = 3112

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    LIVNESL 7
            L+VNESL
Sbjct: 2709 LLVNESL 2715
>NP_775099
          Length = 2595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    LIVNESL 7
            L++NESL
Sbjct: 2205 LLINESL 2211
>NP_056472
          Length = 2277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    LIVNESL 7
            L++NESL
Sbjct: 1887 LLINESL 1893
>NP_001274442
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 219 VVNAALGQ 226
>NP_003911
          Length = 1208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    LIVNESLGQ 9
            ++ NE+LGQ
Sbjct: 1000 VLSNENLGQ 1008
>NP_056416
          Length = 1049

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 603 VNEKMGQ 609
>NP_001138392
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 324 VVNAALGQ 331
>NP_079202
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 324 VVNAALGQ 331
>NP_001274441
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 193 VVNAALGQ 200
>NP_001138395
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 293 VVNAALGQ 300
>NP_001265115
          Length = 794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 348 VNEKMGQ 354
>NP_001265114
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 603 VNEKMGQ 609
>XP_006717816
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 603 VNEKMGQ 609
>XP_006717815
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 482 VNEKMGQ 488
>XP_006717814
          Length = 1057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 603 VNEKMGQ 609
>XP_005250665
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 184 VVNAALGQ 191
>XP_006716198
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVNESLGQ 9
           +VN +LGQ
Sbjct: 218 VVNAALGQ 225
>NP_071362
          Length = 1057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNESLGQ 9
           VNE +GQ
Sbjct: 603 VNEKMGQ 609
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.136    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 418,201
Number of extensions: 644
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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