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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_689417                                                              18    1935
XP_006720248                                                           18    1935
XP_005267848                                                           18    1935
NP_060413                                                              18    1935
NP_443099                                                              17    4312
XP_006720008                                                           17    4312
XP_006720007                                                           17    4312
XP_006720006                                                           17    4312
NP_001006623                                                           15    9605
NP_839955                                                              15    9605
NP_001020095                                                           15    9605
XP_005265807                                                           15    9605
XP_006714105                                                           15    9605
XP_005265805                                                           15    9605
XP_005265804                                                           15    9605
NP_060731                                                              15    9605

>NP_689417
          Length = 711

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IMIKLFRL 9
           IM+K+FRL
Sbjct: 582 IMVKIFRL 589



>XP_006720248
          Length = 711

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IMIKLFRL 9
           IM+K+FRL
Sbjct: 582 IMVKIFRL 589



>XP_005267848
          Length = 711

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IMIKLFRL 9
           IM+K+FRL
Sbjct: 582 IMVKIFRL 589



>NP_060413
          Length = 711

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IMIKLFRL 9
           IM+K+FRL
Sbjct: 582 IMVKIFRL 589



>NP_443099
          Length = 1738

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LIMIKLFRL 9
           LIMI+ FR+
Sbjct: 148 LIMIRAFRI 156



>XP_006720008
          Length = 1645

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LIMIKLFRL 9
           LIMI+ FR+
Sbjct: 148 LIMIRAFRI 156



>XP_006720007
          Length = 1709

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LIMIKLFRL 9
           LIMI+ FR+
Sbjct: 148 LIMIRAFRI 156



>XP_006720006
          Length = 1713

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LIMIKLFRL 9
           LIMI+ FR+
Sbjct: 148 LIMIRAFRI 156



>NP_001006623
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   LIMIKLF 7
           ++M+KLF
Sbjct: 221 IVMVKLF 227



>NP_839955
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 567 MMIKIFR 573



>NP_001020095
          Length = 894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 582 MMIKIFR 588



>XP_005265807
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 567 MMIKIFR 573



>XP_006714105
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 585 MMIKIFR 591



>XP_005265805
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 600 MMIKIFR 606



>XP_005265804
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 600 MMIKIFR 606



>NP_060731
          Length = 894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IMIKLFR 8
           +MIK+FR
Sbjct: 582 MMIKIFR 588



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.366    0.178    0.466 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 250,389
Number of extensions: 212
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.3 bits)
S2: 28 (15.4 bits)