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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     869
NP_444254                                                              19     869
NP_444253                                                              19     869
NP_444255                                                              19     869
XP_005247551                                                           19     869
XP_005247550                                                           19     869
XP_006713712                                                           19     869
XP_005247549                                                           19     869
NP_001129242                                                           17    2528
NP_001129241                                                           17    2528
NP_001129240                                                           17    2528
NP_006496                                                              17    2528
NP_037514                                                              17    3301
NP_001073296                                                           16    5631
NP_003728                                                              16    7355
NP_542454                                                              16    7355
NP_065928                                                              16    7355
NP_060910                                                              16    7355
XP_005256527                                                           16    7355
XP_005253264                                                           16    7355
XP_006716525                                                           16    7355
XP_006716524                                                           16    7355
NP_775791                                                              16    7355
NP_150285                                                              15    9605
NP_054777                                                              15    9605
NP_597713                                                              15    9605
NP_001166171                                                           15    9605
XP_006714597                                                           15    9605
XP_005271944                                                           15    9605
XP_005271943                                                           15    9605
XP_006714596                                                           15    9605
XP_005271942                                                           15    9605

>NP_444256
          Length = 1794

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1247 LGSRQAQV 1254



>NP_444254
          Length = 1845

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1247 LGSRQAQV 1254



>NP_444253
          Length = 1914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1316 LGSRQAQV 1323



>NP_444255
          Length = 1863

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1316 LGSRQAQV 1323



>XP_005247551
          Length = 1855

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1257 LGSRQAQV 1264



>XP_005247550
          Length = 1856

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1326 LGSRQAQV 1333



>XP_006713712
          Length = 1923

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1326 LGSRQAQV 1333



>XP_005247549
          Length = 1924

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    LGSRQAQV 8
            LGSRQAQV
Sbjct: 1326 LGSRQAQV 1333



>NP_001129242
          Length = 392

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LGSRQAQVT 9
           LG+RQA++T
Sbjct: 318 LGARQAELT 326



>NP_001129241
          Length = 364

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LGSRQAQVT 9
           LG+RQA++T
Sbjct: 318 LGARQAELT 326



>NP_001129240
          Length = 372

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LGSRQAQVT 9
           LG+RQA++T
Sbjct: 318 LGARQAELT 326



>NP_006496
          Length = 417

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   LGSRQAQVT 9
           LG+RQA++T
Sbjct: 318 LGARQAELT 326



>NP_037514
          Length = 750

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LGSRQAQVT 9
           +G+RQ QVT
Sbjct: 366 IGARQQQVT 374



>NP_001073296
          Length = 232

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LGSRQAQVT 9
          LG RQ+Q T
Sbjct: 76 LGGRQSQFT 84



>NP_003728
          Length = 3298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LGSRQAQVT 9
            LGS  AQVT
Sbjct: 2291 LGSEIAQVT 2299



>NP_542454
          Length = 195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  GSRQAQ 7
          GSRQAQ
Sbjct: 9  GSRQAQ 14



>NP_065928
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GSRQAQ 7
           GSRQAQ
Sbjct: 328 GSRQAQ 333



>NP_060910
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LGSRQAQVT 9
          LGS Q Q+T
Sbjct: 8  LGSGQMQIT 16



>XP_005256527
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GSRQAQ 7
           GSRQAQ
Sbjct: 328 GSRQAQ 333



>XP_005253264
          Length = 3443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    LGSRQAQVT 9
            LGS  AQVT
Sbjct: 2436 LGSEIAQVT 2444



>XP_006716525
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LGSRQAQVT 9
          LGS Q Q+T
Sbjct: 8  LGSGQMQIT 16



>XP_006716524
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LGSRQAQVT 9
          LGS Q Q+T
Sbjct: 8  LGSGQMQIT 16



>NP_775791
          Length = 152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  LGSRQAQ 7
          LGSRQA+
Sbjct: 50 LGSRQAR 56



>NP_150285
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   LGSRQAQV 8
           LG+R+A+V
Sbjct: 334 LGAREAEV 341



>NP_054777
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RQAQVT 9
           RQAQVT
Sbjct: 330 RQAQVT 335



>NP_597713
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



>NP_001166171
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



>XP_006714597
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



>XP_005271944
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



>XP_005271943
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



>XP_006714596
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



>XP_005271942
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGSRQAQVT 9
           LG R  QVT
Sbjct: 426 LGQRTGQVT 434



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.119    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 397,884
Number of extensions: 422
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)