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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     509
XP_006726167                                                           18    1135
XP_006726072                                                           18    1135
XP_006725979                                                           18    1135
XP_006725887                                                           18    1135
XP_006725770                                                           18    1135
XP_006725556                                                           18    1135
XP_006715213                                                           18    1135
NP_055717                                                              17    3301
XP_005260386                                                           17    3301
NP_443171                                                              17    4312
NP_112572                                                              17    4312
XP_006711210                                                           17    4312
XP_006711209                                                           17    4312
XP_005244929                                                           17    4312
XP_006711208                                                           17    4312
NP_612429                                                              16    5631
NP_001258547                                                           16    5631
NP_060077                                                              16    5631
XP_005259070                                                           16    5631
XP_005267356                                                           16    5631
NP_001137357                                                           16    7355
NP_001137358                                                           16    7355
NP_001231921                                                           16    7355
NP_001231919                                                           16    7355
NP_002471                                                              16    7355
NP_542194                                                              16    7355
NP_002271                                                              16    7355
XP_005268950                                                           16    7355
XP_005268949                                                           16    7355
XP_005268948                                                           16    7355
XP_005268946                                                           16    7355
XP_005268944                                                           16    7355
XP_005268945                                                           16    7355
XP_005268943                                                           16    7355
XP_005268942                                                           16    7355
NP_689488                                                              16    7355

>NP_115803
          Length = 1156

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LGRQSQRST 9
           LG+QSQRST
Sbjct: 344 LGQQSQRST 352



>XP_006726167
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>XP_006726072
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>XP_006725979
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>XP_006725887
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>XP_006725770
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>XP_006725556
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>XP_006715213
          Length = 156

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1 LGRQSQRST 9
         +GR SQRST
Sbjct: 1 MGRSSQRST 9



>NP_055717
          Length = 989

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGRQ++RS
Sbjct: 230 LGRQAERS 237



>XP_005260386
          Length = 992

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGRQ++RS
Sbjct: 230 LGRQAERS 237



>NP_443171
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGR++QRS
Sbjct: 331 LGRKTQRS 338



>NP_112572
          Length = 515

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGR++QRS
Sbjct: 332 LGRKTQRS 339



>XP_006711210
          Length = 485

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  LGRQSQRS 8
          LGR++QRS
Sbjct: 82 LGRKTQRS 89



>XP_006711209
          Length = 613

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGR++QRS
Sbjct: 331 LGRKTQRS 338



>XP_005244929
          Length = 647

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGR++QRS
Sbjct: 244 LGRKTQRS 251



>XP_006711208
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   LGRQSQRS 8
           LGR++QRS
Sbjct: 331 LGRKTQRS 338



>NP_612429
          Length = 5795

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GRQSQR 7
           GRQSQR
Sbjct: 251 GRQSQR 256



>NP_001258547
          Length = 780

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LGRQSQRST 9
           L RQS+RST
Sbjct: 551 LMRQSRRST 559



>NP_060077
          Length = 782

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LGRQSQRST 9
           L RQS+RST
Sbjct: 552 LMRQSRRST 560



>XP_005259070
          Length = 781

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   LGRQSQRST 9
           L RQS+RST
Sbjct: 552 LMRQSRRST 560



>XP_005267356
          Length = 5695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GRQSQR 7
           GRQSQR
Sbjct: 151 GRQSQR 156



>NP_001137357
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>NP_001137358
          Length = 943

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 603 RQSRRST 609



>NP_001231921
          Length = 974

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 634 RQSRRST 640



>NP_001231919
          Length = 995

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>NP_002471
          Length = 1030

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>NP_542194
          Length = 1661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    GRQSQRS 8
            GRQ QRS
Sbjct: 1235 GRQMQRS 1241



>NP_002271
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LGRQSQRST 9
          LGR S R+T
Sbjct: 56 LGRSSYRAT 64



>XP_005268950
          Length = 931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 575 RQSRRST 581



>XP_005268949
          Length = 987

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 631 RQSRRST 637



>XP_005268948
          Length = 990

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 634 RQSRRST 640



>XP_005268946
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>XP_005268944
          Length = 1005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>XP_005268945
          Length = 1005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>XP_005268943
          Length = 1034

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 678 RQSRRST 684



>XP_005268942
          Length = 1046

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RQSQRST 9
           RQS+RST
Sbjct: 690 RQSRRST 696



>NP_689488
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  LGRQSQRS 8
          LGR+ QRS
Sbjct: 34 LGRELQRS 41



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.116    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 401,684
Number of extensions: 555
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)