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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              19     665
NP_005552                                                              17    4312
XP_005258838                                                           17    4312
NP_001078949                                                           17    4312
NP_775904                                                              17    4312
NP_001350                                                              16    5631
NP_000525                                                              16    7355
NP_001121615                                                           16    7355
NP_001121616                                                           16    7355
NP_001827                                                              16    7355
NP_588609                                                              16    7355
NP_001257933                                                           16    7355
NP_003769                                                              16    7355
NP_997708                                                              16    7355
XP_005256281                                                           16    7355
XP_005256280                                                           16    7355
XP_005256278                                                           16    7355
XP_005256277                                                           16    7355
XP_005247914                                                           16    7355
XP_006713864                                                           16    7355
XP_006713863                                                           16    7355
XP_006713862                                                           16    7355
XP_005247912                                                           16    7355
XP_006713861                                                           16    7355
XP_005246706                                                           16    7355
XP_005246704                                                           16    7355
NP_055628                                                              15    9605
NP_002178                                                              15    9605
NP_694985                                                              15    9605
NP_006738                                                              15    9605
NP_001074011                                                           15    9605
XP_005274246                                                           15    9605

>NP_003290
          Length = 803

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   LGRGMTITL 9
           LGRG TITL
Sbjct: 241 LGRGTTITL 249



>NP_005552
          Length = 417

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GRGMTITL 9
           GRG T+TL
Sbjct: 95  GRGHTLTL 102



>XP_005258838
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 275 LGKGFTVT 282



>NP_001078949
          Length = 162

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LGRGMTI 7
          LGRG+T+
Sbjct: 11 LGRGLTV 17



>NP_775904
          Length = 493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 261 LGKGFTVT 268



>NP_001350
          Length = 335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  LGRGMTITL 9
          LG+GMT  L
Sbjct: 71 LGKGMTTLL 79



>NP_000525
          Length = 932

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 137 LGQGTTVT 144



>NP_001121615
          Length = 893

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 137 LGQGTTVT 144



>NP_001121616
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 137 LGQGTTVT 144



>NP_001827
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  GRGMTITL 9
          GR +T+TL
Sbjct: 69 GRSITVTL 76



>NP_588609
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LGRGMTITL 9
          LG+G+ +TL
Sbjct: 15 LGQGLLLTL 23



>NP_001257933
          Length = 186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GMTITL 9
           GMTITL
Sbjct: 168 GMTITL 173



>NP_003769
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>NP_997708
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_005256281
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LGRGMTITL 9
          LG+G+ +TL
Sbjct: 15 LGQGLLLTL 23



>XP_005256280
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LGRGMTITL 9
          LG+G+ +TL
Sbjct: 15 LGQGLLLTL 23



>XP_005256278
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LGRGMTITL 9
          LG+G+ +TL
Sbjct: 15 LGQGLLLTL 23



>XP_005256277
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  LGRGMTITL 9
          LG+G+ +TL
Sbjct: 15 LGQGLLLTL 23



>XP_005247914
          Length = 246

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_006713864
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_006713863
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_006713862
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_005247912
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_006713861
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  LGRGMTIT 8
          LG+G T+T
Sbjct: 57 LGKGKTLT 64



>XP_005246706
          Length = 891

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 137 LGQGTTVT 144



>XP_005246704
          Length = 932

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   LGRGMTIT 8
           LG+G T+T
Sbjct: 137 LGQGTTVT 144



>NP_055628
          Length = 1065

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RGMTITL 9
           RGM +TL
Sbjct: 511 RGMNVTL 517



>NP_002178
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LGRGMTITL 9
          LG G T+T+
Sbjct: 69 LGSGKTLTI 77



>NP_694985
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GRGMTITL 9
           GRG  ITL
Sbjct: 652 GRGEAITL 659



>NP_006738
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GRGMTITL 9
           GRG  ITL
Sbjct: 652 GRGEAITL 659



>NP_001074011
          Length = 1133

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   LGRGMTITL 9
           LGRGM   L
Sbjct: 245 LGRGMATKL 253



>XP_005274246
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GRGMTITL 9
           GRG  ITL
Sbjct: 652 GRGEAITL 659



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.149    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 483,736
Number of extensions: 598
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)