Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001120936                                                           17    2528
NP_001001709                                                           17    3301
NP_001138573                                                           17    3301
XP_006718727                                                           17    3301
NP_116251                                                              17    3301
XP_005276251                                                           17    4312
XP_005245763                                                           17    4312
NP_001842                                                              16    5631
NP_511040                                                              16    5631
XP_006715394                                                           16    5631
XP_005272081                                                           16    5631
XP_006710156                                                           16    5631
XP_006710243                                                           16    5631
NP_060135                                                              16    5631
NP_004237                                                              16    7355
XP_005256918                                                           16    7355
NP_115499                                                              15    9605
NP_954648                                                              15    9605
NP_954649                                                              15    9605
NP_542410                                                              15    9605
NP_542411                                                              15    9605
NP_542412                                                              15    9605
NP_001132914                                                           15    9605
NP_919228                                                              15    9605
NP_001161206                                                           15    9605
NP_001245258                                                           15    9605
NP_001245260                                                           15    9605
NP_055128                                                              15    9605
NP_001139192                                                           15    9605
NP_001161205                                                           15    9605
NP_065175                                                              15    9605
NP_057323                                                              15    9605
NP_001245259                                                           15    9605
NP_818932                                                              15    9605
NP_036288                                                              15    9605
XP_005260382                                                           15    9605
XP_005260381                                                           15    9605
XP_006723804                                                           15    9605
XP_005260380                                                           15    9605
XP_006723803                                                           15    9605
XP_005260379                                                           15    9605
XP_005260378                                                           15    9605
XP_005260377                                                           15    9605
XP_006723802                                                           15    9605
XP_005260376                                                           15    9605
XP_006723801                                                           15    9605
XP_006723800                                                           15    9605
XP_006723799                                                           15    9605
XP_005256732                                                           15    9605
XP_006717031                                                           15    9605
XP_006717030                                                           15    9605
XP_006717029                                                           15    9605
>NP_001120936
          Length = 3925

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    GETGAQR 8
            GETGAQ+
Sbjct: 2689 GETGAQK 2695
>NP_001001709
          Length = 121

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  GETGAQRK 9
          G TG QRK
Sbjct: 25 GRTGGQRK 32
>NP_001138573
          Length = 712

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LGETGAQRK 9
           LG+TG  RK
Sbjct: 329 LGKTGGSRK 337
>XP_006718727
          Length = 712

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   LGETGAQRK 9
           LG+TG  RK
Sbjct: 329 LGKTGGSRK 337
>NP_116251
          Length = 642

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GETGAQRK 9
           GE G QRK
Sbjct: 107 GEVGTQRK 114
>XP_005276251
          Length = 219

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LGETGAQRK 9
           LG++GA+R+
Sbjct: 97  LGDSGARRR 105
>XP_005245763
          Length = 219

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   LGETGAQRK 9
           LG++GA+R+
Sbjct: 97  LGDSGARRR 105
>NP_001842
          Length = 921

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 456 LGEVGAQ 462
>NP_511040
          Length = 678

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 213 LGEVGAQ 219
>XP_006715394
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 162 LGEVGAQ 168
>XP_005272081
          Length = 192

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LGETGAQRK 9
          +GET  +RK
Sbjct: 91 IGETNVERK 99
>XP_006710156
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LGETGA 6
           LGETGA
Sbjct: 164 LGETGA 169
>XP_006710243
          Length = 98

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  LGETGA 6
          LGETGA
Sbjct: 56 LGETGA 61
>NP_060135
          Length = 170

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LGETGAQRK 9
          +GET  +RK
Sbjct: 69 IGETNVERK 77
>NP_004237
          Length = 553

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 510 LGELGAQ 516
>XP_005256918
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 357 LGELGAQ 363
>NP_115499
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 491 LGEGGAQ 497
>NP_954648
          Length = 583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 441 LGEGGAQ 447
>NP_954649
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   LGETGAQ 7
           LGE GAQ
Sbjct: 491 LGEGGAQ 497
>NP_542410
          Length = 1629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GETGAQ 7
           G+TGAQ
Sbjct: 467 GDTGAQ 472
>NP_542411
          Length = 1736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GETGAQ 7
           G+TGAQ
Sbjct: 574 GDTGAQ 579
>NP_542412
          Length = 1650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GETGAQ 7
           G+TGAQ
Sbjct: 488 GDTGAQ 493
>NP_001132914
          Length = 800

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE G+QR
Sbjct: 211 GENGSQR 217
>NP_919228
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE G+QR
Sbjct: 211 GENGSQR 217
>NP_001161206
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    GETGAQRK 9
            GE G QR+
Sbjct: 1051 GEAGTQRQ 1058
>NP_001245258
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>NP_001245260
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 397 GESGGQR 403
>NP_055128
          Length = 157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  LGETGAQ 7
          LG+TGA+
Sbjct: 88 LGQTGAR 94
>NP_001139192
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  LGETGAQRK 9
          LGE   QRK
Sbjct: 11 LGELNEQRK 19
>NP_001161205
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GETGAQRK 9
           GE G QR+
Sbjct: 881 GEAGTQRQ 888
>NP_065175
          Length = 1063

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    GETGAQRK 9
            GE G QR+
Sbjct: 1021 GEAGTQRQ 1028
>NP_057323
          Length = 3530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    GETGAQR 8
            GET AQR
Sbjct: 2360 GETEAQR 2366
>NP_001245259
          Length = 701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 428 GESGGQR 434
>NP_818932
          Length = 779

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 397 GESGGQR 403
>NP_036288
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_005260382
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_005260381
          Length = 744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_006723804
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 397 GESGGQR 403
>XP_005260380
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_006723803
          Length = 853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_005260379
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 458 GESGGQR 464
>XP_005260378
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_005260377
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_006723802
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 458 GESGGQR 464
>XP_005260376
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_006723801
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_006723800
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_006723799
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE+G QR
Sbjct: 459 GESGGQR 465
>XP_005256732
          Length = 3531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    GETGAQR 8
            GET AQR
Sbjct: 2361 GETEAQR 2367
>XP_006717031
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE G+QR
Sbjct: 121 GENGSQR 127
>XP_006717030
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE G+QR
Sbjct: 121 GENGSQR 127
>XP_006717029
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GETGAQR 8
           GE G+QR
Sbjct: 121 GENGSQR 127
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.128    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 610,590
Number of extensions: 1424
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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