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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000236                                                              21     229
NP_001120972                                                           21     229
XP_006716052                                                           21     229
XP_006716051                                                           21     229
NP_002943                                                              17    2528
NP_071351                                                              17    4312
NP_997244                                                              16    5631
NP_060021                                                              16    5631
NP_001145                                                              16    5631
NP_733828                                                              16    5631
NP_001244066                                                           16    5631
NP_851782                                                              16    5631
XP_006717867                                                           16    5631
XP_005262782                                                           16    5631
XP_006714141                                                           16    5631
XP_005262780                                                           16    5631
XP_005262779                                                           16    5631
NP_001185498                                                           16    5631
NP_001685                                                              16    5631
NP_001229679                                                           16    5631
NP_057218                                                              16    7355
XP_005250812                                                           16    7355
NP_997235                                                              15    9605
NP_001726                                                              15    9605
NP_001119595                                                           15    9605
NP_001167560                                                           15    9605
NP_114423                                                              15    9605
NP_001167561                                                           15    9605
NP_059982                                                              15    9605
NP_974487                                                              15    9605
XP_005260914                                                           15    9605
XP_005260913                                                           15    9605
XP_005258175                                                           15    9605
XP_006714247                                                           15    9605
XP_005262992                                                           15    9605

>NP_000236
          Length = 1390

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LGASLNDDI 9
           +GASLNDDI
Sbjct: 333 IGASLNDDI 341



>NP_001120972
          Length = 1408

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LGASLNDDI 9
           +GASLNDDI
Sbjct: 333 IGASLNDDI 341



>XP_006716052
          Length = 1409

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LGASLNDDI 9
           +GASLNDDI
Sbjct: 352 IGASLNDDI 360



>XP_006716051
          Length = 1427

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   LGASLNDDI 9
           +GASLNDDI
Sbjct: 352 IGASLNDDI 360



>NP_002943
          Length = 293

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   LGASLNDDI 9
           LGASL D++
Sbjct: 98  LGASLKDEV 106



>NP_071351
          Length = 2752

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GASLNDDI 9
            GAS ND+I
Sbjct: 1201 GASFNDNI 1208



>NP_997244
          Length = 3169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    SLNDDI 9
            SLNDDI
Sbjct: 1097 SLNDDI 1102



>NP_060021
          Length = 1009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SLNDDI 9
          SLNDDI
Sbjct: 49 SLNDDI 54



>NP_001145
          Length = 320

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           G+SL DD+
Sbjct: 134 GSSLEDDV 141



>NP_733828
          Length = 1322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GAS +DDI
Sbjct: 927 GASYSDDI 934



>NP_001244066
          Length = 903

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GASLNDD 8
           GAS NDD
Sbjct: 200 GASQNDD 206



>NP_851782
          Length = 1283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GAS +DDI
Sbjct: 903 GASYSDDI 910



>XP_006717867
          Length = 896

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SLNDDI 9
          SLNDDI
Sbjct: 49 SLNDDI 54



>XP_005262782
          Length = 1216

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GAS +DDI
Sbjct: 829 GASYSDDI 836



>XP_006714141
          Length = 1259

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GAS +DDI
Sbjct: 872 GASYSDDI 879



>XP_005262780
          Length = 1307

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GAS +DDI
Sbjct: 927 GASYSDDI 934



>XP_005262779
          Length = 1314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GAS +DDI
Sbjct: 927 GASYSDDI 934



>NP_001185498
          Length = 155

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SLNDDI 9
          SLNDDI
Sbjct: 79 SLNDDI 84



>NP_001685
          Length = 155

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SLNDDI 9
          SLNDDI
Sbjct: 79 SLNDDI 84



>NP_001229679
          Length = 245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  LGASLND 7
          LGA LND
Sbjct: 88 LGAELND 94



>NP_057218
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GASL DD+
Sbjct: 419 GASLLDDL 426



>XP_005250812
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GASLNDDI 9
           GASL DD+
Sbjct: 419 GASLLDDL 426



>NP_997235
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   LGASLNDD 8
           +GA  NDD
Sbjct: 209 IGAQSNDD 216



>NP_001726
          Length = 1676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SLNDDI 9
           SLNDD+
Sbjct: 147 SLNDDL 152



>NP_001119595
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LGASLNDDI 9
           +GA L DD+
Sbjct: 141 VGADLQDDM 149



>NP_001167560
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LGASLN 6
           LGASLN
Sbjct: 524 LGASLN 529



>NP_114423
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LGASLN 6
           LGASLN
Sbjct: 540 LGASLN 545



>NP_001167561
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LGASLN 6
           LGASLN
Sbjct: 567 LGASLN 572



>NP_059982
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LGASLNDDI 9
           +GA L DD+
Sbjct: 223 VGADLQDDM 231



>NP_974487
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   LGASLNDDI 9
           +GA L DD+
Sbjct: 244 VGADLQDDM 252



>XP_005260914
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LGASLN 6
           LGASLN
Sbjct: 505 LGASLN 510



>XP_005260913
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   LGASLN 6
           LGASLN
Sbjct: 647 LGASLN 652



>XP_005258175
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  LGASLNDDI 9
          +GA L DD+
Sbjct: 42 VGADLQDDM 50



>XP_006714247
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  LGASLNDD 8
          +GA  NDD
Sbjct: 77 IGAQSNDD 84



>XP_005262992
          Length = 524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   LGASLNDD 8
           +GA  NDD
Sbjct: 209 IGAQSNDD 216



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.139    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 562,336
Number of extensions: 828
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)